The NH2 tail of the novel histone variant H2BFWT exhibits properties distinct from conventional H2B with respect to the assembly of mitotic chromosomes
The NH2 tail of the novel histone variant H2BFWT exhibits properties distinct from conventional H2B with respect to the assembly of mitotic chromosomes
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DOI：
10.1128/mcb.26.4.1518-1526.2006
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发表时间：
2006-02-01
影响因子：
5.3
通讯作者：
Dimitrov, S
中科院分区：
文献类型：
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作者：
Boulard, M;Gautier, T;Dimitrov, S
我们研究了与H_2BFWT重组的核小体的功能和结构性质，H_2BFWT是最近发现的一种功能完全未知的H_2B家族的组蛋白变体。我们发现，在核小体中，H_2BFWT可以取代传统的组蛋白H_2B。H_2BFWT的存在不影响核小体的整体结构，H_2BFWT核小体表现出与常规核小体相同的稳定性。SWI/SNF能有效地改造和动员H_2BFWT核小体。重要的是，与传统的H_2B相比，H_2BFWT不能招募染色体凝集因子，也不能参与有丝分裂染色体的组装。这是由H_2BFWT的高度发散(与常规H_2B相比)的NH_2尾巴决定的。这些数据，结合其他人在精子核中发现的H2BFWT似乎与端粒染色质相关的观察结果，表明H2BFWT可能作为一种特异的表观遗传学标记。
We have studied the functional and structural properties of nucleosomes reconstituted with H2BFWT, a recently identified putative histone variant of the H2B family with totally unknown function. We show that H2BFWT can replace the conventional histone H2B in the nucleosome. The presence of H2BFWT did not affect the overall structure of the nucleosome, and the H2BFWT nucleosomes exhibited the same stability as conventional nucleosomes. SWI/SNF was able to efficiently remodel and mobilize the H2BFWT nucleosomes. Importantly, H2BFWT, in contrast to conventional H2B, was unable to recruit chromosome condensation factors and to participate in the assembly of mitotic chromosomes. This was determined by the highly divergent (compared to conventional H2B) NH2 tail of H2BFWT. These data, in combination with the observations that H2BFWT was found by others in the sperm nuclei and appeared to be associated with the telomeric chromatin, suggest that H2BFWT could act as a specific epigenetic marker.