E2F is required to prevent inappropriate S-phase entry of mammalian cells
E2F is required to prevent inappropriate S-phase entry of mammalian cells
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DOI：
10.1128/mcb.20.1.363-371.2000
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发表时间：
2000-01-01
影响因子：
5.3
通讯作者：
Weintraub, SJ
中科院分区：
文献类型：
--
作者：
He, S;Cook, BL;Weintraub, SJ
E2F 是调节细胞周期的转录因子家族。人们普遍认为，E2F 介导的一组基因的反式激活是控制细胞从 G(1) 期进入 S 期的关键活动。与这一假设相反，我们证明，当细胞被伽马射线照射抑制时，E2F 实际上抑制了两种细胞类型中 S 期的开始。我们的研究结果表明，在这些细胞中，触发从 G(0)/G(1) 停滞进展到 S 期的关键事件是 E2F 介导的细胞周期基因反式抑制的释放，而不是 E2F 的反式激活。此外，我们的数据表明 E2F 介导的反式激活对于这些细胞中的 G(1)/S 相转变不是必需的。
E2F is a family of transcription factors that regulates the cell cycle. It is widely accepted that E2F-mediated transactivation of a set of genes is the critical activity that governs cellular progression through G(1) into S phase. In contrast to this hypothesis, we demonstrate that E2F actually suppresses the onset of S phase in two cell types when the cells are arrested by gamma irradiation. Our findings indicate that in these cells, the critical event triggering progression from G(0)/G(1) arrest into S phase is the release of E2F-mediated transrepression of cell cycle genes, not transactivation by E2F. Furthermore, our data suggest that E2F-mediated transactivation is not necessary for the G(1)/S-phase transition in these cells.