Motility and fertility of rabbit sperm cryopreserved using soybean lecithin as an alternative to egg yolk
Motility and fertility of rabbit sperm cryopreserved using soybean lecithin as an alternative to egg yolk
复制标题
DOI：
10.1016/j.theriogenology.2015.06.018
复制
发表时间：
2015-10-15
期刊：
影响因子：
2.8
通讯作者：
Matsuda, Yukihisa
中科院分区：
文献类型：
--
作者：
Nishijima, Kazutoshi;Kitajima, Shuji;Matsuda, Yukihisa
本研究旨在探讨大豆卵磷脂是否可以作为一种替代的冷冻保护剂，以建立一种不需要使用蛋黄的方法来冷冻保存兔菌株。用含20%蛋黄（EYH）、0.5% （Lec-0.5）、1.5% （Lec-1.5）、2.5% （Lec-2.5）、3.5% （Lec-3.5; wt/vol）卵磷脂（IV-S型，>= 30%）的HEPES填充剂冷冻日本大白兔精液，分析解冻后精子的活力。Lec-1.5组精子活力显著高于Lec-2.5和3.5组，与EYH组相当。EYH组和Lec-1.5组经17轮冻融精子人工授精，均有12只兔怀孕（70.6%）并分娩。EYH组产仔33窝，lec1 -1.5组产仔5.1窝。由此可见，大豆卵磷脂可以替代蛋黄作为冻融兔精子的冷冻保护剂，且卵磷脂含量为1.5% （IV-S型，>= 30%）是兔精子冷冻保存的最佳浓度。(C) 2015爱思唯尔公司版权所有。
This study was conducted to investigate whether soy lecithin can be used as an alternative cryoprotectant to establish a procedure that does not require the use of egg yolk to cryopreserve rabbit strains. Semen from Japanese White rabbits was frozen with HEPES extender containing 20% egg yolk (EYH), 0.5% (Lec-0.5), 1.5% (Lec-1.5), 2.5% (Lec-2.5), or 3.5% (Lec-3.5; wt/vol) lecithin (type IV-S, >= 30%), and the motility of thawed sperm was analyzed. The sperm motility in the Lec-1.5 group was significantly higher than that in the Lec-2.5 and 3.5 groups and equivalent to the EYH group. From 17 rounds of artificial insemination with frozen-thawed sperm in the EYH and Lec-1.5 groups, 12 rabbits in both groups were pregnant (70.6%) and delivered offspring. The litter size was 33 in the EYH group and 5.1 in the Lec-1.5 group. These results indicate that soy lecithin can be used as a substitute for egg yolk as a cryoprotectant on the basis of motility and fertility of the frozen-thawed rabbit sperm and that 1.5% lecithin (type IV-S, >= 30%) in the semen extender was the optimum concentration for rabbit sperm cryopreservation. (C) 2015 Elsevier Inc. All rights reserved.