Determination of chlordane in human blood by gas chromatography.
Determination of chlordane in human blood by gas chromatography.
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DOI：
10.1039/an9851000263
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
I. Saito;N. Kawamura;K. Uno;Y. Takeuchi
中科院分区：
文献类型：
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作者：
I. Saito;N. Kawamura;K. Uno;Y. Takeuchi
研究了气相色谱法测定三氯甲烷前有机氯干扰的消除方法。在0 °C下，用浓硫酸和发烟硝酸（1 + 1 V/V）的冷混合物（5 ml）处理三氯甲烷、多氯联苯和有机氯农药15 min。将产物与冷水（100 ml）混合，连续蒸馏（循环蒸汽萃取）90 min，将三氯甲烷萃取到3 ml庚烷中。提取液用乙酸铁还原净化，用气相色谱电子捕获检测器测定。酸处理去除了多氯联苯和有机氯农药，这使峰重叠的三氯甲烷。方法用于人血中氯霉素的测定。血样用水溶血，用丙酮-己烷（1 + 9 V/V）提取，提取液按上述方法处理。加标回收率在88%以上。
A clean-up method for the removal of organochlorine interference prior to the determination of chlordane by gas chromatography was studied. Chlordane, polychlorinated biphenyls and organochlorine pesticides were treated with a cold mixture (5 ml) of concentrated sulphuric acid and fuming nitric acid (1 + 1 V/V) at 0 °C for 15 min. The product was mixed with cold water (100 ml) and submitted to continuous distillation (cyclic steam extraction) for 90 min to extract chlordane into 3 ml of heptane. The extract was cleaned up by reduction with iron in acetic acid and the chlordane was determined by gas chromatography with electron capture detection. The acid treatment removed polychlorinated biphenyls and organochlorine pesticides, which gave peaks overlapping those of chlordane. The method was applied to the determination of chlordane in human blood. The blood sample was haemolysed with water and extracted with acetone-hexane (1 + 9 V/V), and the extract was treated by the above method. The recovery of chlordane added to human blood was above 88%.