DETECTION OF PLASMODIUM-FALCIPARUM INFECTION WITH THE FLUORESCENT DYE, BENZOTHIOCARBOXYPURINE
DETECTION OF PLASMODIUM-FALCIPARUM INFECTION WITH THE FLUORESCENT DYE, BENZOTHIOCARBOXYPURINE
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DOI：
10.4269/ajtmh.1991.44.11
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发表时间：
1991-01-01
影响因子：
3.3
通讯作者：
HINRICHS, DJ
中科院分区：
文献类型：
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作者：
MAKLER, MT;RIES, LK;HINRICHS, DJ
荧光染料苯并硫代羧基嘌呤(BCP)对核酸有强烈的染色作用。这种染料不会穿透存活的白细胞，但会在固定后对这些细胞进行染色。人们还发现，这种染料会染上存活的恶性疟原虫的核酸。我们随后评估了苯并硫代羧基嘌呤在红细胞内对恶性疟原虫的染色，发现红细胞膜对这种染料是自由渗透的，因此恶性疟原虫在红细胞内被染色。这一发现促使在实验室和现场研究中对这种染料进行了深入的分析，作为Giemsa染色的血液涂片的替代品，并作为加强疟疾感染的显微诊断的一种手段。在一项实地研究中，BCP染料可以在相当于Giemsa方法(寄生虫血症从0.01%到30%)的水平上检测新鲜血液中的疟疾。BCP染色程序也可以用于固定标本，尽管在标本制备后失去了差别染色特征。现场盲取标本111份，Giemsa染色薄涂片阴性22份，厚涂片阴性16份，BCP分析阴性16份。我们发现，与用标准Giemsa染色镜检恶性疟原虫相比，BCP染料具有许多优点。这些优势在低寄生虫血症的条件下尤其明显，在染色和评估的速度方面，以及提供一致结果所需的相对较低的培训水平上。
The fluorescent dye benzothiocarboxypurine (BCP) intensely stains nucleic acids. The dye does not penetrate viable white blood cells but does stain these cells following fixation. It has also been found that the dye stains the nucleic acid of viable Plasmodium falciparum. We have subsequently evaluated the staining of P. falciparum by benzothiocarboxypurine within red blood cells and have found that the red blood cell membrane is freely permeable to this dye and consequently P. falciparum is stained within the red blood cell. This finding prompted an in-depth analysis of the dye in the laboratory and in a field study as an alternative to Giemsa-stained blood smears and as a means of enhancing the microscopic diagnosis of malarial infection. In a field study the BCP dye allowed detection of malaria in fresh blood at a level equivalent to the Giemsa method (parasitemia ranged from 0.01% to 30%). The BCP staining procedure could also be used with fixed specimens although the differential staining characteristics were lost following specimen preparation. Of 111 blinded samples obtained in the field 22 were negative by Giemsa-stained thin smear, 16 were negative on thick smear and the same 16 were negative by BCP analysis. We have found that the BCP dye offers many advantages compared with the microscopic diagnosis of P. falciparum infection with standard Giemsa stains. These advantages are especially evident in conditions of low parasitemia, in the speed of staining and evaluation, and the relatively low level of training required to provide consistent results.