Circulating Protein Fragments of Cartilage and Connective Tissue Degradation Are Diagnostic and Prognostic Markers of Rheumatoid Arthritis and Ankylosing Spondylitis
Circulating Protein Fragments of Cartilage and Connective Tissue Degradation Are Diagnostic and Prognostic Markers of Rheumatoid Arthritis and Ankylosing Spondylitis
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DOI：
10.1371/journal.pone.0054504
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发表时间：
2013-01-24
期刊：
影响因子：
3.7
通讯作者：
Maksymowych, Walter P.
中科院分区：
文献类型：
--
作者：
Bay-Jensen, Anne C.;Wichuk, Stephanie;Maksymowych, Walter P.
炎症驱动的结缔组织更新是类风湿疾病，如强直性脊柱炎（as）的关键。很少有生物标志物可用于测量疾病预后或在这些组织相关疾病中应用的干预措施的有效性。II型胶原是软骨的主要结构蛋白和结缔组织的III型胶原，是胶原溶解的明显靶点，在组织炎症时胶原蛋白增加。本研究的目的是探讨软骨（C2M）和滑膜（C3M）转化生物标志物在AS中的诊断和预后价值。从AS （n = 103）、RA （n = 47）和健康对照（n = 56）患者中提取血清样本。AS进展者被定义为出现新的椎体综合征或两年期间mSASSS变化超过3个单位。C2M ELISA检测血清中II型胶原降解标志物，C3M ELISA检测血清中III型胶原降解标志物。采用Logistic回归和二分类决策树分析各指标单独或联合的预后价值。RA患者C2M和C3M水平均显著高于健康对照组(p
Inflammation driven connective tissue turnover is key in rheumatic diseases, such as ankylosing spondylitis (AS). Few biomarkers are available for measuring disease prognosis or the efficacy of interventions applied in these tissue-related conditions. Type II collagen is the primary structural protein of cartilage and type III collagen of connective tissues, and obvious targets for the collagenalytic, which increase during tissue inflammation. The objective of the study was to investigate the diagnostic and prognostic utility of cartilage, C2M, and synovial, C3M, turnover biomarkers in AS. Serum samples were retrieved from patients suffering from AS (n = 103), RA (n = 47) and healthy controls (n = 56). AS progressors were defined as having new vertebral syndesmophytes or more that 3 unit change in mSASSS over a two-year period. Type II collagen degradation markers in serum were measured by the C2M ELISA, and type III collagen degradation by the C3M ELISA. Logistic regression and dichotomized decision tree were used to analyze the prognostic value of the markers individually or in combination. Both C2M and C3M levels were significantly higher in RA patients than in healthy controls (p