SEX-DIFFERENCES IN VULNERABILITY TO DEVELOPMENTAL SPATIAL-LEARNING DEFICITS INDUCED BY LIMITED BINGE ALCOHOL EXPOSURE IN NEONATAL RATS
SEX-DIFFERENCES IN VULNERABILITY TO DEVELOPMENTAL SPATIAL-LEARNING DEFICITS INDUCED BY LIMITED BINGE ALCOHOL EXPOSURE IN NEONATAL RATS
复制标题
DOI：
10.1006/nlme.1995.0009
复制
发表时间：
1995-11-01
影响因子：
2.7
通讯作者：
PETERSON, SD
中科院分区：
文献类型：
--
作者：
GOODLETT, CR;PETERSON, SD
本研究的两个主要目的是：(1)重复先前的研究结果，即在新生儿大脑发育突增期间，6天的酗酒会导致幼年大鼠出现明显的位置学习缺陷；(2)确定在新生儿时期，更有限的（3天）酗酒是否会导致位置学习缺陷，以及这种影响是否取决于暴露的发育时间。采用人工饲养方法和分窝实验设计，在产后4-6天、7-9天或4-9天给雄性和雌性新生大鼠组大量暴露于4.5 g/kg/天的配方奶中乙醇，其血液酒精浓度平均峰值为230-260 mg/dl。对照组为人工饲养的胃造口对照组（GC），在PD 4-9日饲喂等热量配方奶饲料；从PD 26开始连续6天训练Morris空间导航任务的位置学习习得；在第六天结束时进行了一次探查试验。正如预期的那样，与SC和GC组相比，在PD 4-9组中给予酒精的男性和女性在获取和探测试验表现上都有显著的缺陷。暴露于PD - 7-9的男性有明显的地方学习缺陷，其严重程度与暴露于PD - 7-9的男性相同。在男性中，PD - 4暴露只产生了缓慢习得的不显著趋势。3天暴露对雌性没有显著影响。新生儿大脑发育突增的后期似乎构成了一个性别特异性时期，对酒精诱发的发育性空间学习缺陷的脆弱性增强。(C) 1995学术出版社，Inc。
The two main objectives of this study were (1) to replicate previous findings that 6 days of binge-like exposure to alcohol during the neonatal brain growth spurt induces significant place learning deficits in juvenile rats and (2) to determine whether more limited (3-day) binge-like exposure during the neonatal period induces place learning deficits and whether the effects depend on the developmental timing of the exposure. Using artificial rearing methods and a split-litter experimental design, groups of male and female neonatal rats were given binge-like exposure to 4.5 g/kg/day of ethanol in milk-formula either on Postnatal Days (PD) 4-6, PD 7-9, or PD 4-9, which yielded mean peak blood alcohol concentrations of 230-260 mg/dl. Controls included an artificially reared gastrostomy control group (GC) given an isocaloric milk formula diet on PD 4-9 and a suckle control group reared normally by lactating dams. Acquisition of place learning in the Morris spatial navigation task was trained for 6 consecutive days beginning on PD 26; a probe trial was given at the end of the sixth day. As expected, both males and females given alcohol on PD 4-9 had significant deficits in acquisition and probe trial performance relative to SC and GC groups. Males given the PD 7-9 exposure had significant place learning deficits which were as severe as with the full B-day exposure. The PD 4-6 exposure in males produced only a nonsignificant trend toward slower acquisition. Females were not significantly affected by either 3-day exposure. The latter phase of the neonatal brain growth spurt appears to constitute a sex-specific period of enhanced vulnerability to alcohol-induced developmental spatial learning deficits. (C) 1995 Academic Press, Inc.