Longitudinal European surveillance study of antibiotic resistance of Haemophilus influenzae.
Longitudinal European surveillance study of antibiotic resistance of Haemophilus influenzae.
复制标题
欧洲流感嗜血杆菌抗生素耐药性纵向监测研究。
DOI：
10.1093/jac/dkl310
复制
发表时间：
2006
期刊：
影响因子：
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通讯作者：
D. Milatovic
中科院分区：
文献类型：
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作者：
Wouter T. M. Jansen;A. Verel;M. Beitsma;J. Verhoef;D. Milatovic
目标
我们评估了目前欧洲流感嗜血杆菌对β-内酰胺类和其他药物的耐药率，并将结果与我们以前进行的监测研究进行了比较。
方法
使用肉汤微量稀释法测定抗生素的MIC。对β-内酰胺酶（BL）阴性、阿莫西林耐药（BLNAR）分离株PBP 3的青霉素结合结构域进行测序。
结果
BL阳性和BLNAR菌株的百分比分别为0%至17.6%和0%至33.9%。与1997/98年和2002/03年相比，对阿莫西林不敏感的菌株的总体百分比分别从19.8%和23.3%下降到2004/05年的16.4%。产BL菌株的百分比分别从11.0%和13.7%下降到7.6%，而BLNAR菌株的数量保持稳定（分别为8.8%和9.6%，而2004/05年为8.8%）。青霉素结合蛋白（PBP）3B基因序列BLNAR和敏感株之间的比较发现新的氨基酸突变。
结论
尽管地区间差异很大，但H。在欧洲，由于产BL菌株的数量减少，流感病毒对阿莫西林的敏感性似乎下降，而BLNAR菌株的总体百分比似乎相对恒定。
OBJECTIVES
We assessed the current resistance rates of Haemophilus influenzae against beta-lactams and other agents in Europe and compared the results with those of our previously performed surveillance study.
METHODS
MICs of the antibiotics were determined using broth microdilution. The penicillin-binding domain of PBP3 of beta-lactamase (BL)-negative, amoxicillin-resistant (BLNAR) isolates was sequenced.
RESULTS
The percentage of BL-positive and BLNAR strains ranged from 0% to 17.6% and 0% to 33.9%, respectively. Compared with 1997/98 and 2002/03, the overall percentage of strains non-susceptible to amoxicillin decreased from 19.8% and 23.3%, respectively, to 16.4% in 2004/05. The percentage of BL-producing strains decreased from 11.0% and 13.7%, respectively, to 7.6%, whereas the number of BLNAR strains remained stable (8.8% and 9.6%, respectively, versus 8.8% in 2004/05). Comparison of penicillin binding protein (PBP) 3B gene sequences between BLNAR and susceptible strains revealed novel amino acid mutations.
CONCLUSIONS
In spite of large inter-regional differences, the overall resistance of H. influenzae to amoxicillin in Europe seems to decline due to a decreasing number of BL-producing strains, whereas the overall percentage of BLNAR strains seems relatively constant.
脑膜炎患者中不产生 β-内酰胺酶、耐氨苄西林 b 型流感嗜血杆菌的患病率迅速增加。
DOI：
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发表时间：
2004
期刊：
Antimicrob Agents Chemother 48
影响因子：
--
作者：
Hasegawa K;Chiba N;Kobayashi R;Murayama SY;Iwata S;Sunakawa K;Ubukata K
通讯作者：
Ubukata K