Separation and determination of four artificial sweeteners and citric acid by high-performance anion-exchange chromatography
Separation and determination of four artificial sweeteners and citric acid by high-performance anion-exchange chromatography
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DOI：
10.1016/s0021-9673(97)00067-8
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发表时间：
1997-05-30
影响因子：
4.1
通讯作者：
Ni, ZM
中科院分区：
文献类型：
--
作者：
Chen, QC;Mou, SF;Ni, ZM
建立了一次注射同时分离测定4种人工甜味剂(糖精钠、阿斯巴甜、甜蜜素和安赛蜜-K)和柠檬酸的高效阴离子交换层析方法。采用阴离子交换梯度程序分离糖精钠、阿斯巴甜、安赛蜜-K，电导法测定糖精钠、甜蜜素、安赛蜜-K和柠檬酸。方法的检出限(信噪比3：1)分别为：糖精钠0.019 mU g/ml，阿斯巴甜0.035 mU g/ml，安赛蜜-K 0.044 mU g/ml，糖精0.26mU g/ml，甜蜜素0.16mU g/ml，安赛蜜-K 0.23mU g/ml，柠檬酸0.22mU g/ml。方法已成功地用于饮料和粉状台面甜味剂中4种甜味剂和柠檬酸的测定，各样品的平均回收率为93~107%。
A high-performance anion-exchange chromatographic method for the simultaneous separation and determination of four artificial sweeteners (sodium saccharin, aspartame, sodium cyclamate and acesulfame-K) and citric acid in a single injection is proposed. The separation was performed by an anion-exchange gradient program and determination by ultraviolet absorbance detection for sodium saccharin, aspartame, acesulfame-K in combination with conductivity detection for sodium saccharin, sodium cyclamate, acesulfame-K as well as citric acid in series. Good linearities between the concentrations of all analytes and relevant peak area responses were achieved over the range 2-100 mu g/ml. The detection limits (signal-to-noise ratio 3:1) were 0.019 mu g/ml for sodium saccharin, 0.035 mu g/ml for aspartame, 0.044 mu g/ml for acesulfame-K by ultraviolet absorbance detection and 0.26 mu g/ml for sodium saccharin, 0.16 mu g/ml for sodium cyclamate, 0.23 mu g/ml for acesulfame-K and 0.22 mu g/ml for citric acid by conductivity detection. The method has been successfully applied to the determination of the four sweeteners and citric acid in drinks and powdered tabletop sweeteners, and the average recoveries for various samples ranged from 93 to 107%.