Wechselwirkung des Vanadyl(IV)-Kations mit Nucleotiden in wäßriger Lösung / Interaction of the Vanadyl(IV) Cation with Nucleotides in Aqueous Solution
Wechselwirkung des Vanadyl(IV)-Kations mit Nucleotiden in wäßriger Lösung / Interaction of the Vanadyl(IV) Cation with Nucleotides in Aqueous Solution
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Wechselwirkung des Vanadyl(IV)-Kations mit Nucleotides in wäßriger Lösung / 水溶液中氧钒 (IV) 阳离子与核苷酸的相互作用
DOI：
10.1515/znb-1987-1208
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
E. Baran
中科院分区：
文献类型：
--
作者：
G. Urretavizcaya;E. Baran
摘要在pH = 4.9的条件下，用分光光度法研究了VO2+与单核苷酸、二核苷酸和三核苷酸以及一些相关核苷和碱基的相互作用。结果表明阳离子与所有核苷酸的磷酸基团有优先相互作用，而与核苷和碱基没有相互作用。钒基配位球在单核苷酸的情况下表现出一定的扭曲作用，而在二核苷酸和三核苷酸的情况下则表现得更为规则。在高ph值下，阳离子与去质子化的oh -基团的核糖相互作用。用纯核糖和核糖-5′-磷酸直接测定证实了这种相互作用，而胸腺嘧啶核苷酸则不存在这种相互作用，其中核糖被2′-脱氧核糖所取代。
Abstract The interaction of VO2+ with mono-, di- and trinucleotides and with some of the related nucleosides and bases, was investigated spectrophotometrically at pH = 4.9. The results point to a preferential interaction of the cation with the phosphate groups of all the nucleotides whereas no interaction with the nucleosides and bases was detected. The vanadyl coordination sphere shows some distortive effects in the case of mononucleotides whereas it appears more regular in the case of di- and trinucleotides. At high pH-values the cation interacts with the deprotonated OH-groups of the ribose moieties. This type of interaction was confirmed by direct measurements with pure ribose and ribose-5′-phosphate, and it was absent in the case of thymidine nucleotides, in which ribose is replaced by 2′-deoxyribose.