Different virulence levels of the species of Sporothrix in a murine model
Different virulence levels of the species of Sporothrix in a murine model
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DOI：
10.1111/j.1469-0691.2009.02824.x
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发表时间：
2009-07-01
影响因子：
14.2
通讯作者：
Guarro, J.
中科院分区：
文献类型：
--
作者：
Arrillaga-Moncrieff, I.;Capilla, J.;Guarro, J.
采用免疫活性小鼠模型对临床感兴趣的五种孢子丝菌（白色孢子丝菌、巴西孢子丝菌、球形孢子丝菌、墨西哥孢子丝菌和严格意义上的申克孢子丝菌）的实验致病性进行了比较研究。通过静脉接种小鼠（每组10只），对每种菌种的两种菌株和每种菌株的两种接种水平（2 x 10（7）和2 x 10（4）分生孢子/动物）进行检测。死亡是由于一株S. brasiliensis，而S. brasiliensis和S.申克氏菌株其他接种物和其他测试物种没有杀死任何实验动物。组织负荷研究显示真菌扩散至肾脏、肺、脾、脑和睾丸。S. brasiliensis广泛地从所有研究的器官中回收，S. schenckii和S. globosa的回收量较低。组织学研究揭示了病变的差异，其范围从局部炎症，在感染S的小鼠的注射部位有少量的真菌细胞。globosa感染者的器官中大量真菌细胞浸润。巴西的。结果表明，S. brasiliensis和S. schenckii是毒性最强的物种，表明病变机制可能是物种特异性的。
P>A comparative study on the experimental pathogenicity of five species of Sporothrix of clinical interest, Sporothrix albicans, Sporothrix brasiliensis, Sporothrix globosa, Sporothrix mexicana, and Sporothrix schenckii sensu stricto, was performed using an immunocompetent murine model. Two strains of each species and two levels of inoculum for each strain (2 x 10(7) and 2 x 10(4) conidia/animal) were tested by intravenous inoculation of mice (ten per group). Mortality was caused by the low inoculum of one strain of S. brasiliensis only, and the high inocula of S. brasiliensis and S. schenckii strains. Other inocula and other species tested did not kill any of the experimental animals. Tissue burden studies showed fungal spread to kidneys, lungs, spleen, brain, and testicles. S. brasiliensis was recovered extensively from all of the studied organs, and S. schenckii and S. globosa were recovered in lower amounts. Histopathological studies revealed differences in the lesions, which ranged from local inflammation with a low number of fungal cells at the injection site in mice infected with S. globosa, to massive infiltration of fungal cells in organs of those infected with S. brasiliensis. Our findings showed that S. brasiliensis and S. schenckii were the most virulent species, and suggest that lesional mechanisms could be species-specific.