Short UV-B Exposure Stimulated Enzymatic and Nonenzymatic Antioxidants and Reduced Oxidative Stress of Cold-Stored Mangoes
Short UV-B Exposure Stimulated Enzymatic and Nonenzymatic Antioxidants and Reduced Oxidative Stress of Cold-Stored Mangoes
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短时间 UV-B 暴露刺激酶和非酶抗氧化剂并降低冷藏芒果的氧化应激
DOI：
10.1021/acs.jafc.5b04460
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发表时间：
2015-12-30
影响因子：
6.1
通讯作者：
Xu, Maojun
中科院分区：
文献类型：
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作者：
Jiang, Zhifang;Zheng, Yaoqi;Xu, Maojun
研究了UV-B辐射对冷藏芒果活性氧水平、抗氧化物质含量、抗氧化酶活性和氧化损伤的影响。经5 KJm（-2）UV-B辐射4 h后，冷藏果实超氧阴离子产生速率、过氧化氢浓度、离子渗漏水平和丙二醛含量均显著低于对照果实。与对照相比，UV-B辐射处理的芒果中活性氧产生酶活性没有变化，但超氧化物歧化酶和过氧化氢酶活性、抗坏血酸和多酚含量以及抗氧化能力显著提高。UV-B增强的抗氧化物质和抗氧化酶与UV-B辐射芒果中ROS水平的降低高度相关。这些数据表明，短的UV-B暴露减少氧化应激和减轻氧化损伤的冷藏芒果触发酶和非酶抗氧化系统，虽然在水果中的ROS生成没有受到影响。
The effects of UV-B irradiation on reactive oxygen species (ROS) levels, antioxidant compound contents, antioxidative enzyme activities, and oxidative damage of cold-stored mangoes were examined. Superoxide anion production rate, hydrogen peroxide concentration, ion leakage level and malondialdehyde content of the cold-stored fruit preradiated with 5 KJ m(-2) UV-B for 4 h were significantly decreased as compared with control fruit. The activities of ROS generating enzymes remained unchanged in UV-B-irradiated mangoes as compared to the control, but superoxide dismutase and catalase activities, ascorbate and polyphenol contents and antioxidant capacities of the cold-stored mangoes were significantly enhanced by UV-B. The UV-B-enhanced antioxidant compounds and antioxidative enzymes were highly correlated with the reduced-ROS levels in UV-B-irradiated mangoes. The data indicated that a short UV-B exposure reduced oxidative stress and alleviated oxidative damage of the cold-stored mangoes by triggering both enzymatic and nonenzymatic antioxidant systems although ROS generation in the fruit was not affected.