Conversion by Peyer's patch lymphocytes of human enterocytes into M cells that transport bacteria
Conversion by Peyer's patch lymphocytes of human enterocytes into M cells that transport bacteria
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DOI：
10.1126/science.277.5328.949
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发表时间：
1997-08-15
期刊：
影响因子：
56.9
通讯作者：
Pringault, E
中科院分区：
文献类型：
--
作者：
Kerneis, S;Bogdanova, A;Pringault, E
排列在肠道上的上皮是大分子和微生物不渗透的，但在佩耶氏斑（PPs）中，淋巴样滤泡相关上皮（FAE）含有运输抗原和微生物的M细胞。用分化的人肠细胞系Caco-2培养PP淋巴细胞，建立了具有FAE和M细胞主要特征的培养体系。淋巴细胞进入上皮单层，诱导刷状边界的重组以及颗粒和霍乱弧菌的温度依赖性运输。该模型系统可用于肠道生理学、疫苗研究和药物传递研究。
The epithelium that lines the gut is impermeable to macromolecules and microorganisms, except in Peyer's patches (PPs), where the lymphoid follicle-associated epithelium (FAE) contains M cells that transport antigens and microorganisms. A cultured system that reproduces the main characteristics of FAE and M cells was established by cultivation of PP lymphocytes with the differentiated human intestinal cell line Caco-2. Lymphocytes settled into the epithelial monolayer, inducing reorganization of the brush border and a temperature-dependent transport of particles and Vibrio cholerae. This model system could prove useful for intestinal physiology, vaccine research, and drug delivery studies.