CIRCULATING GLUCAGON AFTER TOTAL PANCREATECTOMY IN MAN
CIRCULATING GLUCAGON AFTER TOTAL PANCREATECTOMY IN MAN
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DOI：
10.1007/bf00282517
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发表时间：
1983-01-01
期刊：
影响因子：
8.2
通讯作者：
STADIL, F
中科院分区：
文献类型：
--
作者：
HOLST, JJ;PEDERSEN, JH;STADIL, F
In 5 totally pancreatectomized human subjects the secretion of gut-derived glucagons was stimulated by ingestion of a meal rich in fat and carbohydrates. Glucagon-like immunoreactivity in plasma, measured with an antiserum against the 6-15 sequence, increased 5-fold in response to the meal. Glucagon like immunoreactivity measured with an antiserum against the C-terminal sequence was initially normal (12-13 pmol/l), increased slightly (to 20 pmol/l), and then decreased (to .apprx. 6 pmol/l) The chromatographic profile of glucagon-like immunoreactivity in plasma at maximal stimulation was studied after concentration by affinity chromatography. Both assay systems identified 2 peaks (at Kd-values of 0.30 and 0.60-0.65, 0.30 and 0.70, respectively). The position at Kd 0.70 corresponds to that of glucagon 1-29. The same components may be identified in plasma from normal subjects. The human intestine apparently is capable of generating all of the molecular forms of glucagon which normally are present in plasma.
