Differential expression of estrogen receptor alpha and beta in rat dorsal root ganglion neurons
Differential expression of estrogen receptor alpha and beta in rat dorsal root ganglion neurons
复制标题
DOI：
10.1002/(sici)1097-4547(19990901)57:5
复制
发表时间：
1999-09-01
影响因子：
4.2
通讯作者：
Oblinger, MM
中科院分区：
文献类型：
--
作者：
Taleghany, N;Sarajari, S;Oblinger, MM
雌激素受体α（ERα）和雌激素受体β（ERβ）mRNA均在大鼠背根神经节（DRG）神经元中表达，但这两种mRNA的分布明显不同。对 ER α 或 ER β mRNA 具有高度特异性的放射性标记探针用于原位杂交研究；使用两种针对 ER α 蛋白的特异性抗体进行免疫细胞化学，并使用特异性引物进行逆转录聚合酶链反应（RT-PCR）研究，结果表明 ER β mRNA 在雄性和雌性大鼠的 DRG 中广泛表达，几乎所有神经元都显示阳性信号。相反，ER α mRNA 以及核定位的 ER α 蛋白的定位受到更多限制，存在于许多（但不是全部）小型 (< 600 μ m(2)) DRG 神经元中，但很少存在于较大的神经元中。L6-S1 DRG 水平（包含支配生殖组织的感觉神经元）与 L3-L5 DRG 水平相比，ER α 相对丰富，L3-L5 DRG 水平包含支配生殖组织的感觉神经元。与去势对照组相比，对去势大鼠进行长期雌激素治疗（21-28天）显着降低了 DRG 中免疫细胞化学可检测到的 ER α 蛋白，RT-PCR 研究还表明，对去势大鼠进行长期雌激素治疗下调了 ER α mRNA 的水平，但上调了 DRG 中 ER β mRNA 的水平。循环雌性大鼠中，发情前期DRG中的ERα和ERβmRNA水平均高于发情后期。这些发现表明，在发情周期期间动态发生的雌激素受体表达变化不同于卵巢切除动物的长期雌激素治疗所引起的变化。 J. Neurosci，Res，57:603-615，1999。(C) 1999 Wiley-Liss, Inc.
Estrogen receptor alpha (ER alpha) and estrogen receptor beta (ER beta) mRNAs are both expressed in rat dorsal root ganglion (DRG) neurons, but the distribution of these two mRNAs differs markedly. Radiolabeled probes highly specific to ER alpha or ER beta mRNAs were used for in situ hybridization studies; two antibodies specific to ER alpha protein were used for immunocytochemistry and specific primers were used for reverse transcription polymerase chain reaction (RT-PCR) studies, These revealed that ER beta mRNA is widely expressed in the DRG of both male and female rats, with virtually all neurons showing positive signals. In contrast, ER alpha mRNA, as well as nuclear localized ER alpha protein, is more restricted in its localization and is present in many, but not all, of the small-sized (< 600 mu m(2)) DRG neurons, but is only rarely present in larger neurons, The L6-S1 DRG levels, which contain sensory neurons that innervate reproductive tissues, are relatively enriched in ER alpha compared to L3-L5 DRG levels, which contain sensory neurons that innervate hind limb regions, Long-term estrogen treatment of ovariectomized rats (21-28 days) dramatically reduces immunocytochemically detectable ER alpha protein in the DRG relative to that in ovariectomized controls, RT-PCR studies also showed that long-term estrogen treatment of ovariectomized rats downregulates the levels of ER alpha mRNA, but upregulates the levels of ER beta mRNA in the DRG, Interestingly, in intact cycling female rats, ER alpha and ER beta mRNA levels in the DRG were both higher during proestrus compared to metestrus, These findings suggest that the changes in expression of estrogen receptors which occur dynamically during the estrus cycle differ from those induced by long-term estrogen treatment of ovariectomized animals. J. Neurosci, Res, 57:603-615, 1999. (C) 1999 Wiley-Liss, Inc.