Vascular endothelial growth factor promotes brain tissue regeneration with a novel biomaterial polydimethylsiloxane–tetraethoxysilane
Vascular endothelial growth factor promotes brain tissue regeneration with a novel biomaterial polydimethylsiloxane–tetraethoxysilane
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DOI：
10.1016/j.brainres.2006.09.117
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发表时间：
2007-02
期刊：
影响因子：
2.9
通讯作者：
Hanzhe Zhang;Takeshi Hayashi;K. Tsuru;K. Deguchi;Mitsuyuki Nagahara;S. Hayakawa;M. Nagai;T. Kamiy
中科院分区：
文献类型：
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作者：
Hanzhe Zhang;Takeshi Hayashi;K. Tsuru;K. Deguchi;Mitsuyuki Nagahara;S. Hayakawa;M. Nagai;T. Kamiy
在梗塞或创伤后的大脑中，组织最终变得全坏死并形成空腔。在这种情况下，植入或迁移的细胞需要支架来产生新组织。因此，在本研究中，我们尝试使用一种新型生物材料——聚二甲基硅氧烷-四乙氧基硅烷（PDMS-TEOS）混合物来恢复脑组织，其中含有或不含有血管内皮生长因子（VEGF），这对于新血管的形成至关重要。当PDMS-TEOS支架植入人工脑缺损时，它保留在植入部位并保持脑形状的完整性。植入后 30 天，PDMS-TEOS 支架的边缘区域被新形成的组织占据。免疫组织化学分析显示，新组织由星形胶质细胞和内皮细胞组成。添加VEGF增加了新产生的组织体积，免疫组织化学分析显示星形胶质细胞和内皮细胞的数量增加。增殖标记物 Ki67 双重染色表明 VEGF 显着增加了新形成的星形胶质细胞和内皮细胞，表明 VEGF 的添加加速了组织修复和血管生成。这些结果表明，植入具有 VEGF 的 PDMS-TEOS 支架可能对治疗陈旧性脑梗塞或创伤有效。
In the brain after infarction or trauma, the tissue eventually becomes pannecrotic and forms a cavity. In such situations, a scaffold is necessary for the implanted or migrated cells to produce new tissue. In this present study, therefore, we attempted to restore brain tissue using a novel biomaterial, polydimethylsiloxane–tetraethoxysilane (PDMS-TEOS) hybrid with or without vascular endothelial growth factor (VEGF), which is crucial for new vessel formation. When PDMS-TEOS scaffold was implanted into the artificial brain defect, it remained at the implanted site and kept the integrity of the brain shape. At 30 days after the implantation, the marginal territory of PDMS-TEOS scaffold became occupied by newly formed tissue. Immunohistochemical analysis revealed that the new tissue was constituted by astrocytes and endothelial cells. Addition of VEGF increased the newly produced tissue volume, and the immunohistochemical analysis showed that the numbers of astrocytes and endothelial cells were increased. Double staining with proliferation maker Ki67 demonstrated that VEGF significantly increased newly formed astrocytes and endothelial cells, indicating that addition of VEGF accelerated tissue restoration and angiogenesis. These findings show that implantation of PDMS-TEOS scaffold with VEGF might be effective for treating old brain infarction or trauma.