Brain-derived neurotrophic factor (BDNF) expression in normal and regenerating olfactory epithelium of Xenopus laevis
Brain-derived neurotrophic factor (BDNF) expression in normal and regenerating olfactory epithelium of Xenopus laevis
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DOI：
10.1016/j.aanat.2014.10.010
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发表时间：
2015-01-01
影响因子：
2.2
通讯作者：
Paz, Dante Agustin
中科院分区：
文献类型：
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作者：
Frontera, Jimena Laura;Cervino, Ailen Soledad;Paz, Dante Agustin
嗅觉上皮具有终身不断再生嗅觉受体神经元的能力。成人神经发生是神经干细胞增殖和分化的结果，因此，嗅觉神经上皮为研究神经再生及其所有细胞类型的维持和再生所涉及的因素提供了极好的机会。我们分析了在正常生理条件下以及化学破坏非洲爪蟾幼虫嗅觉上皮诱导大量再生过程中BDNF在嗅觉系统中的表达。我们描述了BDNF在嗅上皮和嗅球中的表达和存在。在正常生理条件下，嗅觉上皮和嗅球颗粒细胞中的支撑细胞（胶质细胞）和少量分散的基底细胞（干细胞）表达BDNF。此外，在大量再生过程中，我们发现表达BDNF的基底细胞急剧增加，嗅球和神经中的BDNF也急剧增加。这些结果表明BDNF在嗅觉系统的维持和再生中起着重要作用。(C) 2014 Elsevier GmbH版权所有。
Olfactory epithelium has the capability to continuously regenerate olfactory receptor neurons throughout life. Adult neurogenesis results from proliferation and differentiation of neural stem cells, and consequently, olfactory neuroepithelium offers an excellent opportunity to study neural regeneration and the factors involved in the maintenance and regeneration of all their cell types. We analyzed the expression of BDNF in the olfactory system under normal physiological conditions as well as during a massive regeneration induced by chemical destruction of the olfactory epithelium in Xenopus laevis larvae. We described the expression and presence of BDNF in the olfactory epithelium and bulb. In normal physiological conditions, sustentacular (glial) cells and a few scattered basal (stem) cells express BDNF in the olfactory epithelium as well as the granular cells in the olfactory bulb. Moreover, during massive regeneration, we demonstrated a drastic increase in basal cells expressing BDNF as well as an increase in BDNF in the olfactory bulb and nerve. Together these results suggest an important role of BDNF in the maintenance and regeneration of the olfactory system. (C) 2014 Elsevier GmbH. All rights reserved.