Circulating autoantibodies recognize and bind dying neurons following injury to the brain.
Circulating autoantibodies recognize and bind dying neurons following injury to the brain.
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大脑受伤后，循环自身抗体会识别并结合垂死的神经元。
DOI：
10.1093/jnen/61.12.1100
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发表时间：
2002
影响因子：
3.2
通讯作者：
Kalil,RonaldE
中科院分区：
文献类型：
--
作者：
Stein,ThorD;Fedynyshyn,JosephP;Kalil,RonaldE
虽然已知自身免疫细胞可以防止脑创伤后的细胞损伤，但自身抗体在脑损伤中的作用尚不清楚。在这里，我们提出了在成年大鼠大脑皮质损伤后，循环中的免疫球蛋白自身抗体与病变附近的死亡神经元结合的证据。在单侧视皮层损伤后4h至7d，我们观察到损伤附近的神经元呈免疫阳性反应。其中许多免疫球蛋白阳性神经元处于晚期变性阶段。观察到的免疫染色的大小与所使用的抗体对大鼠免疫球蛋白的反应性百分比成正比。抗体与大鼠血清的预吸附消除了免疫染色。此外，皮质损伤切片上的血清白蛋白免疫染色为阴性，支持免疫球蛋白阳性染色不是血清蛋白被动扩散到受损细胞的结论。相反，这里提出的证据强烈表明，自然产生的免疫球蛋白自身抗体与受损大脑中死亡的神经元特异性结合。我们认为这种自身抗体结合可能参与了受损神经元的吞噬和移除。
While it is known that autoimmune cells can protect against cell damage following traumatic injury of the brain, the role of autoantibodies in brain injury is less clear. Here we present evidence in adult rats that following a cortical lesion of the brain, circulating IgG autoantibodies bind to dying neurons in the vicinity of the lesion. At intervals that ranged from 4 h to 7 days after making a unilateral lesion of visual cortex, we observed neurons near the lesion that were immunopositive for rat IgG. Many of these IgG-positive neurons were in advanced stages of degeneration. The magnitude of the immunostaining observed was directly proportional to the percent reactivity to rat IgG of the antibodies that were used. Preadsorption of the antibodies with rat serum eliminated the immunostaining. In addition, immunostaining for serum albumin in sections through the cortical lesion was negative, supporting the conclusion that the positive staining for IgG does not result from the passive diffusion of serum proteins into injured cells. Instead, the evidence presented here strongly suggests that naturally occurring IgG autoantibodies bind specifically to dying neurons in the injured brain. We propose that this autoantibody binding may participate in the phagocytosis and removal of injured neurons.