Rufy3 promotes metastasis through epithelial-mesenchymal transition in colorectal cancer
Rufy3 promotes metastasis through epithelial-mesenchymal transition in colorectal cancer
复制标题
Rufy3通过上皮间质转化促进结直肠癌转移
DOI：
10.1016/j.canlet.2017.01.001
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发表时间：
2017-04-01
期刊：
影响因子：
9.7
通讯作者：
Wang, Jide
中科院分区：
文献类型：
--
作者：
Xie, Ruyi;Wang, Jing;Wang, Jide
Rufy3 is a RUN domain-containing protein that has been associated with gastric cancers; however, the role of Rufy3 in the progression of colorectal cancer (CRC) remains unknown. We demonstrated that Rufy3 expression was high& in 11/12 fresh CRC tissues than in adjacent normal tissues. Rufy3 induced elevated expression and transactivity of four major oncogenes in CRC. Moreover, siRNA-mediated repression of Rufy3 induced G0/G1 cell cycle arrest, and Rufy3 overexpression enhanced CRC cell proliferation in vitro and in vivo. Furthermore, Rufy3 up-regulation promoted epithelial mesenchymal transition (EMT) and metastatic phenotypes. Using an established in vitro cell model of 5-fluorouracilresistant (5-FU) CRC cells, we assessed cellular morphology, molecular changes, and invasion and found that these characteristics were consistent with EMT. Silencing of Rufy3 by siRNA reversed EMT and greatly diminished the invasion of 5-FU-treated cells. In addition, TGF-beta 1 induced Rufy3 expression in a dose-dependent manner, and Rufy3 knockdown inhibited TGF-beta 1-induced EMT. In vivo, higher expression of Rufy3 promoted CRC cell invasion and metastasis and induced EMT. Taken together, this work identified that Rufy3 promoted cancer metastasis in CRC cells through EMT induction. (C) 2017 Elsevier B.V. All rights reserved.
