Donor-delivered cell wall hydrolases facilitate nanotube penetration into recipient bacteria
Donor-delivered cell wall hydrolases facilitate nanotube penetration into recipient bacteria
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DOI：
10.1038/s41467-020-15605-1
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发表时间：
2020-04-22
影响因子：
16.6
通讯作者：
Ben-Yehuda, Sigal
中科院分区：
文献类型：
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作者：
Baidya, Amit K.;Rosenshine, Ilan;Ben-Yehuda, Sigal
细菌可以产生膜状纳米管，介导细菌细胞之间的接触依赖性分子交换。然而，目前还不清楚纳米管如何穿过细胞壁从供体中出现或渗透到受体细胞中。在这里，我们报告说，枯草芽孢杆菌利用细胞壁重塑酶，LytC酰胺酶及其增强剂LytB，有效的纳米管挤出和渗透。在lytBC突变体中，纳米管的产生减少，并且形成的少数纳米管似乎缺乏穿透到靶细胞中。供体衍生的LytB分子定位于沿着纳米管和纳米管连接的相邻细胞的表面上，主要在纳米管渗透的位点。此外，LytB来自供体B。枯草芽孢杆菌可以激活来自不同物种的受体细菌的LytC，促进细胞壁水解以建立纳米管连接。我们的数据提供了一个细胞间连接装置如何在相邻细菌之间形成的机制视图。细菌可以产生膜状纳米管，介导细菌细胞之间的接触依赖性分子交换。在这里，Baidya等人表明，枯草芽孢杆菌的细胞壁重塑酶是有效的纳米管挤出和渗透所必需的，并且可以通过纳米管传递给其他细菌物种。
Bacteria can produce membranous nanotubes that mediate contact-dependent exchange of molecules among bacterial cells. However, it is unclear how nanotubes cross the cell wall to emerge from the donor or to penetrate into the recipient cell. Here, we report that Bacillus subtilis utilizes cell wall remodeling enzymes, the LytC amidase and its enhancer LytB, for efficient nanotube extrusion and penetration. Nanotube production is reduced in a lytBC mutant, and the few nanotubes formed appear deficient in penetrating into target cells. Donor-derived LytB molecules localize along nanotubes and on the surface of nanotube-connected neighbouring cells, primarily at sites of nanotube penetration. Furthermore, LytB from donor B. subtilis can activate LytC of recipient bacteria from diverse species, facilitating cell wall hydrolysis to establish nanotube connection. Our data provide a mechanistic view of how intercellular connecting devices can be formed among neighbouring bacteria. Bacteria can produce membranous nanotubes that mediate contact-dependent exchange of molecules between bacterial cells. Here, Baidya et al. show that cell-wall remodelling enzymes from Bacillus subtilis are required for efficient nanotube extrusion and penetration, and can be delivered to other bacterial species via nanotubes.