Arabidopsis WRKY38 and WRKY62 Transcription Factors Interact with Histone Deacetylase 19 in Basal Defense
Arabidopsis WRKY38 and WRKY62 Transcription Factors Interact with Histone Deacetylase 19 in Basal Defense
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DOI：
10.1105/tpc.107.055566
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发表时间：
2008-09-01
期刊：
影响因子：
11.6
通讯作者：
Chen, Zhixiang
中科院分区：
文献类型：
--
作者：
Kim, Kang-Chang;Lai, Zhibing;Chen, Zhixiang
拟南芥WRKY38和WRKY62编码两个结构相似的III型WRKY转录因子，它们被水杨酸(SA)或毒力紫丁香假单胞菌以依赖于PR基因1(NPR1)的方式诱导。Wrky38和Wrky62单突变体的抗病性和SA调节的致病相关基因(PR1)的表达在较大程度上是在双突变体中增强的。WRKY38或WRKY62的过表达降低了抗病性和PR1的表达。因此，WRKY38和WRKY62作为植物基础防御的负调控因子具有相加的功能。WRKY38和WRKY62与组蛋白脱乙酰酶19(HDA19)相互作用。紫丁香也能诱导HDA19的表达，其诱导转录本的稳定性取决于SA和NPR1在感染植株中的稳定性。HDA19基因的破坏导致抗性降低，而HDA19的过度表达则导致对紫丁香疫霉的抗性增强。因此，HDA19具有与WRKY38和WRKY62相反的基本抗性作用。WRKY38和WRKY62都是植物细胞中的转录激活因子，但它们的激活活性被过表达的HDA19所消除。WRKY38和WRKY62与HDA19的相互作用可能起到微调植物基础防御反应的作用。
Arabidopsis thaliana WRKY38 and WRKY62, encoding two structurally similar type III WRKY transcription factors, are induced in a Nonexpressor of PR Gene1 (NPR1)-dependent manner by salicylic acid ( SA) or by virulent Pseudomonas syringae. Disease resistance and SA-regulated Pathogenesis-Related1 (PR1) gene expression are enhanced in the wrky38 and wrky62 single mutants and, to a greater extent, in the double mutants. Overexpression of WRKY38 or WRKY62 reduces disease resistance and PR1 expression. Thus, WRKY38 and WRKY62 function additively as negative regulators of plant basal defense. WRKY38 and WRKY62 interact with Histone Deacetylase 19 (HDA19). Expression of HDA19 is also induced by P. syringae, and the stability of its induced transcripts depends on SA and NPR1 in infected plants. Disruption of HDA19 leads to compromised resistance, whereas its overexpression results in enhanced resistance to P. syringae. Thus, HDA19 has a role opposite from those of WRKY38 and WRKY62 in basal resistance to the bacterial pathogen. Both WRKY38 and WRKY62 are transcriptional activators in plant cells, but their activation activities are abolished by overexpressed HDA19. Interaction of WRKY38 and WRKY62 with HDA19 may act to fine-tune plant basal defense responses.