Viridot: An automated virus plaque (immunofocus) counter for the measurement of serological neutralizing responses with application to dengue virus
Viridot: An automated virus plaque (immunofocus) counter for the measurement of serological neutralizing responses with application to dengue virus
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DOI：
10.1371/journal.pntd.0006862
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发表时间：
2018-10-01
影响因子：
3.8
通讯作者：
Cummings, Derek A. T.
中科院分区：
文献类型：
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作者：
Katzelnick, Leah C.;Escoto, Ana Coello;Cummings, Derek A. T.
量化对许多病毒（包括登革热病毒（DENV））的中和抗体反应的金标准方法是斑块减少中和试验（PRNT，也称为免疫焦点减少中和试验）。在96孔板上进行的PRNT具有高通量，比在6孔板或24孔板上进行的PRNT需要更小的抗血清体积，但人工斑块计数具有挑战性，现有的自动斑块计数器昂贵或难以优化。我们已经开发了Viridot （Viridot包），这是一个带有用户界面的R程序，可以计算各种表型的病毒斑块，估计中和抗体滴度，并执行病毒学家使用的其他计算。Viridot空斑计数器包括一个自动参数识别模式（遗漏）
The gold-standard method for quantifying neutralizing antibody responses to many viruses, including dengue virus (DENV), is the plaque reduction neutralization test (PRNT, also called the immunofocus reduction neutralization test). The PRNT conducted on 96-well plates is high-throughput and requires a smaller volume of antiserum than on 6- or 24-well plates, but manual plaque counting is challenging and existing automated plaque counters are expensive or difficult to optimize. We have developed Viridot (Viridot package), a program for R with a user interface in shiny, that counts viral plaques of a variety of phenotypes, estimates neutralizing antibody titers, and performs other calculations of use to virologists. The Viridot plaque counter includes an automatic parameter identification mode (misses