Continuous Immunoassay for Sulfamethazine by Surface Plasmon Resonance-Based Biosensor
Continuous Immunoassay for Sulfamethazine by Surface Plasmon Resonance-Based Biosensor
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DOI：
10.1080/00032710903402556
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发表时间：
2010-01
影响因子：
2
通讯作者：
Hui Li;H. Cai;Xing Chen;Jianhai Sun;Lulu Zhang;D. Cui
中科院分区：
文献类型：
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作者：
Hui Li;H. Cai;Xing Chen;Jianhai Sun;Lulu Zhang;D. Cui
在许多表面等离子体共振（SPR）生物传感器测定中，传感器芯片表面的再生是困难的。不适当的再生将缩短传感器芯片的寿命并降低数据的质量。考虑到降低再生频率的优点，建立了一种理论上可行的磺胺二甲嘧啶（SMT）连续SPR生物传感器免疫分析方法。在连续抑制免疫测定中，传感器芯片表面在一定数量的测试后仅再生一次，而不是每次测试。将SMT-牛血清白蛋白（BSA）偶联物共价固定在羧甲基葡聚糖修饰的金膜上。研究了抗原的固定化条件，优化了抗体的工作稀释度。将抗体与用PBS缓冲液制备的不同浓度的SMT混合以构建校准曲线。检测限为0.5 ng mL−1。连续SPR生物传感器检测被证明是简单和更实用的比正常的。
Regeneration of the sensor chip surface is difficult in many surface plasmon resonance (SPR) biosensor assays. Improper regeneration will reduce life span of the sensor chip and decrease the quality of the data. Considering the advantages of reducing the regeneration frequency, a theoretically feasible continuous SPR biosensor immunoassay for sulfamethazine (SMT) was developed. In the continuous inhibitive immunoassay, the sensor chip surface is regenerated only once after a definite number of tests instead of every test. The SMT-bovine serum albumin (BSA) conjugate was covalently immobilized to a carboxymethyldextran modified gold film. The immobilization conditions of the antigen were studied and the working dilution of the antibody was optimized. The antibody was mixed with SMT of different concentrations prepared with PBS buffer to construct the calibration curve. The limit of detection was 0.5 ng mL−1. The continuous SPR biosensor assay was proved to be simpler and more practical than a normal one.