Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri
Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri
复制标题
亚洲柑橘木虱甲壳素脱乙酰酶 3 的免疫功能分析
DOI：
10.3390/ijms21010064
复制
发表时间：
2020-01-01
影响因子：
5.6
通讯作者：
Lu, Zhan-Jun
中科院分区：
文献类型：
--
作者：
Yu, Hai-Zhong;Li, Ning-Yan;Lu, Zhan-Jun
甲壳素脱乙酰酶（Chitin deacetylase，CDA）是一种严格催化甲壳素脱乙酰化生成壳聚糖的甲壳素降解酶，在调节生长发育和免疫应答中起着重要作用。本研究从柑橘木虱基因组数据库中克隆了一个几丁质脱乙酰酶3基因（chitin deacetylase 3，CDA 3），该基因的开放阅读框（open reading frame，ORF）为1362 bp，编码453个氨基酸。时空表达分析表明，D. Citri CDA 3（DcCDA 3）在珠被和三龄若虫期表达量最高。此外，20-羟基蜕皮激素（20 E）可诱导DcCDA 3表达水平。注射大肠杆菌和金黄色葡萄球菌诱导中肠DcCDA 3上调，而脂肪体中DcCDA 3下调。RNA干扰沉默DcCDA 3后，对D.柑橘属表型此外，杀菌试验表明，重组DcCDA 3抑制革兰氏阳性菌，包括S。金黄色葡萄球菌和枯草芽孢杆菌（B. subtilis）。结论：DcCDA 3可能在D.柑橘。
Chitin deacetylase (CDA) is a chitin degradation enzyme that strictly catalyzes the deacetylation of chitin to form chitosan, which plays an important role in regulating growth and development, as well as the immune response. In this study, a chitin deacetylase 3 gene (CDA3) was identified with a complete open reading frame (ORF) of 1362 bp from the genome database of Diaphorina citri, encoding a protein of 453 amino acids. Spatiotemporal expression analysis suggested that D. citri CDA3 (DcCDA3) had the highest expression level in the integument and third-instar nymph stage. Furthermore, DcCDA3 expression level can be induced by 20-hydroxyecdysone (20E). Injection of Escherichia coli and Staphylococcus aureus induced the upregulation of DcCDA3 in the midgut, while DcCDA3 was downregulated in the fat body. After silencing DcCDA3 by RNA interference, there was no influence on the D. citri phenotype. In addition, bactericidal tests showed that recombinant DcCDA3 inhibited gram-positive bacteria, including S. aureus and Bacillus subtilis (B. subtilis). In conclusion, our results suggest that DcCDA3 might play an important role in the immune response of D. citri.