Prediction of chemical carcinogenicity in rodents from in vitro genetic toxicity assays.
Prediction of chemical carcinogenicity in rodents from in vitro genetic toxicity assays.
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DOI：
10.1126/science.3554512
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发表时间：
1987-05
期刊：
影响因子：
56.9
通讯作者：
R. Tennant;B. Margolin;Shelby;E. Zeiger;J. Haseman;J. Spalding;W. Caspary;M. Resnick;S. Stasiewicz;B. Anderson
中科院分区：
文献类型：
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作者：
R. Tennant;B. Margolin;Shelby;E. Zeiger;J. Haseman;J. Spalding;W. Caspary;M. Resnick;S. Stasiewicz;B. Anderson
评价了四种广泛使用的遗传毒性体外试验预测选定化学品对啮齿动物致癌性的能力。这些试验包括沙门氏菌和小鼠淋巴瘤细胞的致突变试验以及中国仓鼠卵巢细胞的染色体畸变和姐妹染色单体交换试验。本评价中使用了最近在国家癌症研究所和国家毒理学计划进行的2年致癌性研究中检测的73种化学品。四项体外试验的试验结果未显示与啮齿动物致癌性结果的个体一致性存在显著差异;每项试验的一致性约为60%。在本研究的范围内，没有证据表明四种检测试剂之间存在互补性，并且从这些检测试剂构建的检测组合对沙门氏菌检测试剂的总体性能没有显著改善。代表一系列三种细胞类型和四个终点的体外试验确实显示出它们之间的实质性一致性，表明在一种体外试验中呈阳性的化学品在其他体外试验中往往也呈阳性。
Four widely used in vitro assays for genetic toxicity were evaluated for their ability to predict the carcinogenicity of selected chemicals in rodents. These assays were mutagenesis in Salmonella and mouse lymphoma cells and chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells. Seventy-three chemicals recently tested in 2-year carcinogenicity studies conducted by the National Cancer Institute and the National Toxicology Program were used in this evaluation. Test results from the four in vitro assays did not show significant differences in individual concordance with the rodent carcinogenicity results; the concordance of each assay was approximately 60 percent. Within the limits of this study there was no evidence of complementarity among the four assays, and no battery of tests constructed from these assays improved substantially on the overall performance of the Salmonella assay. The in vitro assays which represented a range of three cell types and four end points did show substantial agreement among themselves, indicating that chemicals positive in one in vitro assay tended to be positive in the other in vitro assays.