Synchronised Expressions of LPXRFamide Peptide and its Receptor Genes: Seasonal, Diurnal and Circadian Changes During Spawning Period in Grass Puffer
Synchronised Expressions of LPXRFamide Peptide and its Receptor Genes: Seasonal, Diurnal and Circadian Changes During Spawning Period in Grass Puffer
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DOI：
10.1111/j.1365-2826.2010.02081.x
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发表时间：
2011-01-01
影响因子：
3.2
通讯作者：
Ando, H.
中科院分区：
文献类型：
--
作者：
Shahjahan, Md.;Ikegami, T.;Ando, H.
在RF酰胺肽家族中，LPXRF酰胺肽（LPXRFa）组调节各种垂体激素的释放，并且最近发现LPXRFa基因通过褪黑激素受光周期调节。作为研究LPXRFa对在半月周期中产卵的草河豚（Takifugu niphobles）的生殖功能的作用的第一步，克隆了编码LPXRFa及其受体（LPXRFa-R）的基因，并通过定量实时聚合酶链反应检测了脑和垂体中mRNA的绝对量的季节性、昼夜和昼夜变化。草河豚LPXRFa前体含有两个推定的RF酰胺肽和一个可能的RY酰胺肽。LPXRFa和LPXRFa-R基因在间脑和垂体中广泛表达。这两个基因的表达水平显着升高，在产卵期间，在两性的大脑和垂体，虽然他们是低的产卵鱼后，释放卵子和精子。用金鱼LPXRFa处理原代垂体培养物增加了卵泡刺激素β和黄体生成素β亚基mRNA的量。在间脑中，LPXRFa和LPXRFa-R基因表现出同步的昼夜变化和昼夜变化，分别在授时计时间3和昼夜时间15出现一个峰值。LPXRFa和LPXRFa-R基因在间脑和垂体中的相关表达模式以及LPXRFa对促性腺激素亚基基因表达的可能刺激作用表明LPXRFa和LPXRFa-R系统在调节草河豚月同步产卵中的功能意义。
Among the RFamide peptide family, the LPXRFamide peptide (LPXRFa) group regulates the release of various pituitary hormones and, recently, LPXRFa genes were found to be regulated by photoperiod via melatonin. As a first step towards investigating the role of LPXRFa on reproductive function in grass puffer (Takifugu niphobles), which spawns in semilunar cycles, genes encoding LPXRFa and its receptor (LPXRFa-R) were cloned, and seasonal, diurnal and circadian changes in their absolute amounts of mRNAs in the brain and pituitary were examined by quantitative real-time polymerase chain reaction. The grass puffer LPXRFa precursor contains two putative RFamide peptides and one possible RYamide peptide. LPXRFa and LPXRFa-R genes were extensively expressed in the diencephalon and pituitary. The expression levels of both genes were significantly elevated during the spawning periods in both sexes in the brain and pituitary, although they were low in the spawning fish just after releasing eggs and sperm. The treatment of primary pituitary cultures with goldfish LPXRFa increased the amounts of follicle-stimulating hormone beta- and luteinising hormone beta-subunit mRNAs. In the diencephalon, LPXRFa and LPXRFa-R genes showed synchronised diurnal and circadian variations with one peak at zeitgeber time 3 and circadian time 15, respectively. The correlated expression patterns of LPXRFa and LPXRFa-R genes in the diencephalon and pituitary and the possible stimulatory effects of LPXRFa on gonadotrophin subunit gene expression suggest the functional significance of the LPXRFa and LPXRFa-R system in the regulation of lunar-synchronised spawning of grass puffer.