Combined treatment with sustained-release basic fibroblast growth factor and heparin enhances neovascularization in hypercholesterolemic mouse hindlimb ischemia
Combined treatment with sustained-release basic fibroblast growth factor and heparin enhances neovascularization in hypercholesterolemic mouse hindlimb ischemia
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DOI：
10.1253/circj.71.412
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发表时间：
2007-03-01
影响因子：
3.3
通讯作者：
Komeda, Masashi
中科院分区：
文献类型：
--
作者：
Arai, Yoshio;Fujita, Masatoshi;Komeda, Masashi
背景
研究了碱性成纤维细胞生长因子（bFGF）缓释剂与肝素联合治疗是否能增强高胆固醇血症小鼠后肢缺血的新生血管形成。
方法与结果
将野生型C57BL/6小鼠和低密度脂蛋白受体缺陷小鼠分为以下4个实验组中的一组，并在股动脉抽取后治疗2周：N组，不治疗；H组，每日皮下注射肝素钙；F组，单次肌肉注射bFGF缓释微球；FH组，bFGF缓释剂与肝素联合治疗。在股动脉抽取4周后的野生型小鼠中，H组、F组和FH组的激光多普勒灌注图像指数（LDPII）显著高于N组。FH组的血管密度在4组中最高。3个治疗组的成熟指数显著高于N组。在高胆固醇血症小鼠中，FH组的LDPII显著高于其他3组。FH组的血管密度和成熟指数在4组中最高。
结论
bFGF缓释剂与肝素联合治疗可增强高胆固醇血症后肢缺血模型中的新生血管形成。
Background Whether the combined treatment with sustained-release basic fibroblast growth factor (bFGF) and heparin enhances neovascularization in hypercholesterolemic mouse hindlimb ischemia was investigated.Methods and Results Wild-type C57BL/6 and low density lipoprotein receptor-deficient mice were assigned to 1 of the following 4 experimental groups and treated for 2 weeks after femoral artery extraction: group N, no treatment; group H, daily subcutaneous injection of heparin calcium; group F, single intramuscular injection of the sustained-release bFGF microspheres; and group FH, combined treatment with sustained-release bFGF and heparin. Among the wild-type mice at 4 weeks after femoral artery extraction, the laser Doppler perfusion image index (LDPII) in groups H, F, and FH was significantly higher than that in group N. The vascular density in group FH was the highest among the 4 groups. The maturation index in the 3 treated groups was significantly higher than that in group N. Among the hypercholesterolemic mice, the LDPII in group FH was significantly higher than that in the other 3 groups. The vascular density and maturation index in group FH were the highest among the 4 groups.Conclusions Combined treatment with sustained-release bFGF and heparin enhanced neovascularization in the hypercholesterolemic hindlimb ischemia model.