Magnetic resonance and fluorescence imaging of doxorubicin-loaded nanoparticles using a novel in vivo model.
Magnetic resonance and fluorescence imaging of doxorubicin-loaded nanoparticles using a novel in vivo model.
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DOI：
10.1016/j.nano.2010.06.005
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发表时间：
2010-12
影响因子：
5.4
通讯作者：
Makale, Milan
中科院分区：
文献类型：
--
作者：
Erten, Ahmet;Wrasidlo, Wolf;Scadeng, Miriam;Esener, Sadik;Hoffman, Robert M.;Bouvet, Michael;Makale, Milan
我们在这里报告的多功能药物递送纳米粒子的体内组合模态成像。这些基于葡聚糖核的隐形脂质体纳米颗粒（纳米体）含有阿霉素、用于MRI对比的氧化铁和用于荧光的Bodipy。由于用聚乙二醇（PEG）进行表面装饰并掺入了为物理稳定性而进行UV交联的乙酰化脂质，因此颗粒在体内寿命较长。我们开发了一种啮齿动物背侧皮褶窗口室，有助于对相同肿瘤中纳米颗粒积聚进行MRI和非破坏性光学成像。腔室肿瘤用DsRed-2基因标记，使MR图像、肿瘤的红色荧光和纳米颗粒的蓝色荧光能够共定位。然后将用窗口室开发的纳米颗粒设计和MR成像扩展到表达DsRed-2的原位胰腺肿瘤。使用氧化铁-葡聚糖脂质体和荧光对肿瘤进行MR成像，以证明这些纳米颗粒的深度成像能力。
We report here the in vivo combined-modality imaging of multifunctional drug delivery nanoparticles. These dextran core-based stealth liposomal nanoparticles (nanosomes) contained doxorubicin, iron oxide for MRI contrast, and Bodipy for fluorescence. The particles were long-lived in vivo due to surface decoration with polyethylene glycol (PEG) and the incorporation of acetylated lipids which were UV cross-linked for physical stability. We developed a rodent dorsal skinfold window chamber which facilitated both MRI and non-destructive optical imaging of nanoparticle accumulation in the same tumors. Chamber tumors were genetically labeled with DsRed-2 that enabled the MR images, the red fluorescence of the tumor, and the blue fluorescence of the nanoparticles to be co-localized. The nanoparticle design and MR imaging developed with the window chamber were then extended to orthotopic pancreatic tumors expressing DsRed-2. The tumors were MR imaged using iron oxide-dextran liposomes and by fluorescence to demonstrate the deep imaging capability of these nanoparticles.
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影响因子：
3.3
作者：
KOENIG, SH;KELLAR, KE
通讯作者：
KELLAR, KE
DOI：
10.1016/0005-2736(91)90246-5
发表时间：
1991-07-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ALLEN, TM;HANSEN, C;YAUYOUNG, A
通讯作者：
YAUYOUNG, A
DOI：
10.1016/0005-2736(82)90451-5
发表时间：
1982-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
LOPEZ, E;OBRIEN, DF;WHITESIDES, TH
通讯作者：
WHITESIDES, TH
影响因子：
3.9
作者：
BARBARESE, E;HO, SY;SIMON, RH
通讯作者：
SIMON, RH
DOI：
10.1016/0005-2736(91)90337-8
发表时间：
1991-02-11
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
SENIOR, J;DELGADO, C;GREGORIADIS, G
通讯作者：
GREGORIADIS, G