Juvenile hormone biosynthesis in moths: synthesis and evaluation of farnesol homologs as alternate substrates of farnesol oxidase.
Juvenile hormone biosynthesis in moths: synthesis and evaluation of farnesol homologs as alternate substrates of farnesol oxidase.
复制标题
飞蛾中的保幼激素生物合成：法呢醇同系物作为法呢醇氧化酶替代底物的合成和评估。
DOI：
10.1016/s0965-1748(03)00041-9
复制
发表时间：
2003
影响因子：
3.8
通讯作者：
Hannemann,DE
中科院分区：
文献类型：
--
作者：
Sen,SE;Sperry,AE;Childress,M;Hannemann,DE
法尼醇氧化为法尼醇是昆虫幼体激素（JH）生物合成的一个重要步骤，它是由一种或多种位于微小内分泌腺体（allatum）内的醇氧化酶介导的。由于鳞翅目昆虫具有产生同源JH结构的能力，因此通过测定几种萜类化合物抑制飞蛾中金合糖醇氧化的能力，研究了金合糖醇氧化酶的底物选择性。结果表明，倍半萜烯醇链C-3位置的空间体积增加不利于抑制效力。三乙基homofarneol （1h）是jh0的前体，因此是M. sexta胚胎的生理重要代谢物，被发现是一种较差的法尼醇氧化抑制剂，但被氧化的量与法尼醇几乎相同。这些数据表明，香体的法尼醇氧化酶在控制飞蛾JH同源物的产生中起有限的作用，并表明另一种存在于飞蛾发育早期的氧化酶参与了JH同源物的构建。
The oxidation of farnesol to farnesal is an important step in insect juvenile hormone (JH) biosynthesis and is mediated by one or more alcohol oxidases located within the minute endocrine gland, the corpus allatum. Because lepidopteran insects have the capacity to produce homologous JH structures, the substrate selectivity of farnesol oxidase was examined by determining the ability of several terpenol homologs to inhibit farnesol oxidation in moths. Results utilizing corpora allata homogenates from larval, adult, and embryonic Manduca sexta indicate that increased steric bulk at the C-3 position of the sesquiterpenol chain is detrimental to inhibitory potency. Triethylhomofarnesol (1h), which is precursor to JH 0 and therefore a physiologically important metabolite of M. sexta embryos, was found to be a poor inhibitor of farnesol oxidation but was oxidized in almost same amount as farnesol. This data indicate that farnesol oxidase of the corpus allatum plays a limited role in controlling JH homolog production in moths, and suggests that another oxidative enzyme, which is present at early stages of moth development, is involved in JH homolog construction.