Development of standard tests to examine viscoelastic properties of blood of experimental animals for pediatric mechanical support device evaluation
Development of standard tests to examine viscoelastic properties of blood of experimental animals for pediatric mechanical support device evaluation
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DOI：
10.1097/01.mat.0000242248.66083.48
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发表时间：
2006-09-01
期刊：
影响因子：
4.2
通讯作者：
Kameneva, Marina V.
中科院分区：
文献类型：
--
作者：
Marascalco, Philip J.;Ritchie, Sean P.;Kameneva, Marina V.
我们研究了测量牛、羊和猪全血的粘弹性来评估红细胞亚致死性损伤的适用性。血液粘度和弹性增加，而红细胞压积和血浆粘度没有变化，则意味着红细胞变形性降低。血液粘弹性采用Vilastic Science粘弹仪进行评估。由于正常牛羊血中天然不存在RBC聚集，且RBC体积较小，因此不容易检测到粘弹性。然而，我们发现，将血液红细胞压积调整到40-50%的标准水平可以灵敏地评估这些血型的粘弹性，显示出明显的非牛顿行为，主要与红细胞变形性有关。猪血在所有测试的红细胞压积数值上都表现出明显的非牛顿行为，这使得它在流变学上与人血相当。血液暴露在均匀的机械应力下后，粘度和弹性都会增加。经热暴露硬化的红细胞表现出粘弹性随剪切率的变化而丧失。血液粘弹性的测量在牛、羊，特别是猪血中是有意义的，并可用于评估体外和动物心脏辅助装置试验中的亚致死血液损伤。
We investigated the applicability of measuring the viscoelasticity of bovine, ovine, and porcine whole blood for the evaluation of sublethal damage to red blood cells (RBCs). An increase in blood viscosity and elasticity without changes in hematocrit and plasma viscosity would signify a decrease in RBC deformability. Blood viscoelasticity was assessed using a Vilastic Scientific viscoelastometer. Due to the natural absence of RBC aggregation and small RBC size in normal bovine and ovine blood, viscoelastic properties are less readily detected. However, we found that adjustment of blood hematocrit to a standard level of 40-50% allows for sensitive assessment of viscoelasticity in these blood types demonstrating a marked non-Newtonian behavior mostly related to RBC deformability. Porcine blood showed a pronounced non-Newtonian behavior at all tested hematocrit values, which makes it rheologically comparable to human blood. Both viscosity and elasticity were elevated after blood exposure to a uniform mechanical stress. RBCs rigidified by heat exposure demonstrated a loss of viscoelasticity dependence on shear rate. Measurements of blood viscoelasticity can be meaningful in bovine, ovine, and, especially, porcine blood, and can be used for evaluation of sublethal blood damage during in vitro and animal trials of heart-assist devices.