Evaluation of a combination of alfaxalone with medetomidine and butorphanol for inducing surgical anesthesia in laboratory mice
Evaluation of a combination of alfaxalone with medetomidine and butorphanol for inducing surgical anesthesia in laboratory mice
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DOI：
10.14943/jjvr.64.2.131
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发表时间：
2016-05-01
影响因子：
0.4
通讯作者：
Ohsugi, Takeo
中科院分区：
文献类型：
--
作者：
Higuchi, Shota;Yamada, Riku;Ohsugi, Takeo
研究了阿法沙龙对小鼠的麻醉作用。对小鼠单独给予阿法沙龙（100 mg/kg）或0.3 mg/kg美托咪定和5 mg/kg布托啡诺与阿法沙龙的组合，剂量为20 mg/kg（M/B/A20）、40 mg/kg（M/B/A40）、60 mg/kg（M/B/A60）或80 mg/kg（M/B/A80）。对照小鼠接受0.3 mg/kg美托咪定、4 mg/kg咪达唑仑和5 mg/kg布托啡诺（M/M/B）。每种药物通过腹膜内（IP）或皮下（SC）途径给药。M/M/B IP未达到手术麻醉，但M/M/B SC在给药后10 min内达到手术麻醉，并维持麻醉45 min。IP或SC单独给予阿法沙龙后麻醉评分非常低。M/B/A20 IP和SC未实现手术麻醉。M/B/A40 IP未达到手术麻醉，而M/B/A40 SC在给药后10 min内达到手术麻醉并维持麻醉35 min; M/B/A60 SC在给药后5 min内达到手术麻醉并维持麻醉75 min;相比之下，M/B/A60 IP未达到手术麻醉。M/B/A80 SC在给药后5 min内达到手术麻醉，并维持麻醉85 min，而M/B/A80 IP未达到手术麻醉，给药后约10 min有1只小鼠死亡。阿替美唑给药可迅速逆转M/B/A60诱导的小鼠麻醉。这些结果表明，M/B/A60 SC，一种基于阿法沙龙的组合，适用于诱导实验室小鼠的手术麻醉。
The anesthetic effects of alfaxalone were investigated in mice. Mice were administered alfaxalone (100 mg/kg) alone or the combinations of 0.3 mg/kg of medetomidine and 5 mg/kg of butorphanol with alfaxalone at doses of 20 mg/kg (M/B/A20), 40 mg/kg (M/B/A40), 60 mg/kg (M/B/A60), or 80 mg/kg (M/B/A80). Control mice received 0.3 mg/kg of medetomidine, 4 mg/kg of midazolam, and 5 mg/kg of butorphanol (M/M/B). Each drug was administrated by intraperitoneal (IP) or subcutaneous (SC) routes. M/M/B IP did not achieve surgical anesthesia but M/M/B SC achieved surgical anesthesia within 10 min after administration and maintained anesthesia for 45 min. The anesthetic scores were very low after IP or SC administration of alfaxalone alone. M/B/A20 IP and SC did not achieve surgical anesthesia. M/B/A40 IP did not achieve surgical anesthesia but M/B/A40 SC achieved surgical anesthesia within 10 min after administration and maintained anesthesia for 35 min. M/B/A60 SC achieved surgical anesthesia within 5 min after administration and maintained anesthesia for 75 min. By contrast, M/B/A60 IP did not achieve surgical anesthesia. M/B/A80 SC achieved surgical anesthesia within 5 min after administration and maintained anesthesia for 85 min. By contrast, M/B/A80 IP did not achieve surgical anesthesia and one mouse died about 10 min after drug administration. Administration of atipamezole rapidly reversed anesthesia induced by M/B/A60 in mice. These results suggest that M/B/A60 SC, an alfaxalone-based combination, is suitable for inducing surgical anesthesia in laboratory mice.