Knockdown of Long Noncoding RNA LUCAT1 Inhibits Cell Viability and Invasion by Regulating miR-375 in Glioma.
Knockdown of Long Noncoding RNA LUCAT1 Inhibits Cell Viability and Invasion by Regulating miR-375 in Glioma.
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DOI：
10.3727/096504017x15088061795756
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发表时间：
2018-03-05
影响因子：
3.1
通讯作者：
Li LZ
中科院分区：
文献类型：
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作者：
Gao YS;Liu XZ;Zhang YG;Liu XJ;Li LZ
最近，长链非编码RNA（lncRNA）已成为包括胶质瘤在内的几种癌症的新基因调控因子和预后标志物。在这里，我们专注于lncRNA LUCAT 1对胶质瘤的进展。采用qRT-PCR检测LUCAT 1和miR-375在胶质瘤组织和细胞中的表达。MTT法和Transwell侵袭实验检测LUCAT 1在胶质瘤进展中的作用。使用生物信息学工具DIANA预测LUCAT 1的靶点。进行Pearson相关性分析以探索LUCAT 1和miR-375之间的相关性。在本研究中，我们发现LUCAT 1在胶质瘤组织和细胞中显著上调。LUCAT 1抑制显著抑制胶质瘤细胞的增殖和侵袭。随后，DIANA表明，miR-375被预测含有LUCAT 1的互补结合位点。荧光素酶报告基因分析显示miR-375直接靶向LUCAT 1。此外，我们发现miR-375在胶质瘤组织中表达下调，并且与LUCAT 1在胶质瘤组织中的表达呈负相关。此外，结果显示miR-375可以挽救LUCAT 1在胶质瘤进展中的功能。lncRNA LUCAT 1通过调节miR-375对胶质瘤细胞的增殖和侵袭至关重要。我们的研究结果表明，LUCAT 1可能为胶质瘤的治疗提供一个潜在的新的治疗靶点。
Recently, long noncoding RNAs (lncRNAs) have emerged as new gene regulators and prognostic markers in several cancers, including glioma. Here we focused on lncRNA LUCAT1 on the progression of glioma. qRT-PCR was used to determine the expression of LUCAT1 and miR-375 in glioma tissues and cells. MTT and Transwell invasion assays were performed to determine the function of LUCAT1 in glioma progression. The bioinformatics tool DIANA was used to predict the targets of LUCAT1. Pearson’s correlation analysis was performed to explore the correlation between LUCAT1 and miR-375. In the present study, we showed that LUCAT1 was substantially upregulated in glioma tissues and cells. LUCAT1 inhibition significantly suppressed the proliferation and invasion of glioma cells. Subsequently, DIANA showed that miR-375 was predicted to contain the complementary binding sites to LUCAT1. Luciferase reporter assay showed that miR-375 directly targeted LUCAT1. In addition, we found that miR-375 was downregulated in glioma tissues and negatively correlated with LUCAT1 expression in glioma tissues. Furthermore, the results showed that miR-375 could rescue the function of LUCAT1 in glioma progression. The lncRNA LUCAT1 was critical for the proliferation and invasion of glioma cells by regulating miR-375. Our findings indicated that LUCAT1 might offer a potential novel therapeutic target for the treatment of glioma.