CHARACTERIZATION OF STAPHYLOCOCCUS-AUREUS ISOLATES FROM PATIENTS WITH TOXIC SHOCK SYNDROME, USING POLYETHYLENE INFECTION CHAMBERS IN RABBITS
CHARACTERIZATION OF STAPHYLOCOCCUS-AUREUS ISOLATES FROM PATIENTS WITH TOXIC SHOCK SYNDROME, USING POLYETHYLENE INFECTION CHAMBERS IN RABBITS
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DOI：
10.1128/iai.39.1.383-387.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
BEST, GK
中科院分区：
文献类型：
--
作者：
SCOTT, DF;KLING, JM;BEST, GK
本文比较了中毒性休克综合征（TSS）患者分离的金黄色葡萄球菌与非TSS患者分离的金黄色葡萄球菌对家兔的毒力。当将生物体注射到s.c.腔（穿孔聚乙烯高尔夫球）中以评估毒力时，观察到tSS菌株迅速死亡，而非tSS菌株则没有。在16株TSS毒株中，11株对33只家兔造成致死性感染；5个对照菌株中0个造成10只家兔死亡。与TSS菌株相关的增强毒力的证据似乎与接种量的大小无关。尽管在最初的24小时内，原始接种量减少，但产生致命感染的菌株似乎还是这样做了。当在透析牛肉心脏培养基中生长时，所有TSS菌株和非TSS菌株都没有产生具有中性pI[等电点]的细胞外蛋白。TSS菌株和非TSS菌株之间没有其他生理差异。
Isolates of S. aureus from patients with toxic shock syndrome (TSS) were compared with non-TSS strains of S. aureus with respect to their virulence in rabbits. When the organisms were injected into s.c. chambers (perforated polyethylene golf balls) to assess virulence, a rapid mortality was observed with tSS but not with non-TSS strains. Of 16 TSS strains, 11 caused lethal infections in 33 rabbits tested; 0 of 5 control strains caused mortality in 10 rabbits. This evidence of enhanced virulence associated with TSS strains did not appear to be associated with the size of the inoculum. Strains which produced lethal infections appeared to do so despite a reduction in the size of the original inoculum during the first 24 h. All of the TSS strains and none of the non-TSS strains elaborated extracellular protein(s) with a neutral pI [isoelectric point] when grown in a dialyzed beef heart medium. No other physiological difference was noted between the TSS and non-TSS strains.