STRUCTURE AND NOVEL EXONS OF THE HUMAN-TAU GENE
STRUCTURE AND NOVEL EXONS OF THE HUMAN-TAU GENE
复制标题
DOI：
10.1021/bi00158a027
复制
发表时间：
1992-11-03
期刊：
影响因子：
2.9
通讯作者：
KOSIK, KS
中科院分区：
文献类型：
--
作者：
ANDREADIS, A;BROWN, WM;KOSIK, KS
微管结合蛋白 tau 对于神经元形态的建立和维持非常重要，并且是阿尔茨海默氏症大脑神经原纤维缠结 (NFT) 特征的主要组成部分。神经元特异性 tau 转录物经历复杂的选择性剪接。人类 tau 基因已被克隆并绘制图谱。该基因的限制性分析和部分测序表明，它包含（1）四个先前在啮齿动物和牛中描述但在人类 tau cDNA 中没有的可变剪接外显子，以及（2）两个 CpG 岛，一个与启动子区域相关，另一个与外显子 9 相关。对人 tau mRNA 的检查表明，人类大脑皮质剪接模式与先前报道的鼠和牛 tau mRNA 的剪接模式不同，尽管 所有三个基因中都有保守的外显子组织。
The microtubule-binding protein tau is important in establishing and maintaining neuronal morphology and is a major component of the neurofibrillary tangles (NFTs) characteristic of Alzheimer's brain. The neuron-specific tau transcript undergoes complex alternative splicing. The human tau gene has been cloned and mapped. The restriction analysis and partial sequencing of the gene shows that it contains (1) four alternatively spliced exons previously described in rodent and bovine but not in human tau cDNAs and (2) two CpG islands, one associated with the promoter region, the other with exon 9. Examination of human tau mRNA indicates that the human cerebrocortical splicing pattern differs from that previously reported for the murine and bovine tau mRNAs, despite conserved exon organization in all three genes.