Extremely Low Frequency Electromagnetic Fields Facilitate Vesicle Endocytosis by Increasing Presynaptic Calcium Channel Expression at a Central Synapse.
Extremely Low Frequency Electromagnetic Fields Facilitate Vesicle Endocytosis by Increasing Presynaptic Calcium Channel Expression at a Central Synapse.
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极低频电磁场通过增加中央突触的突触前钙通道表达来促进囊泡内吞作用
DOI：
10.1038/srep21774
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发表时间：
2016-02-18
影响因子：
4.6
通讯作者：
Xue L
中科院分区：
文献类型：
--
作者：
Sun ZC;Ge JL;Guo B;Guo J;Hao M;Wu YC;Lin YA;La T;Yao PT;Mei YA;Feng Y;Xue L
越来越多的证据表明，极低频电磁场（ELF-EMF）引起的重大生物效应。虽然外吞作用在神经元通讯中起着重要的物理和生物作用，但关于ELF-EMF如何调节这一过程的研究很少。通过直接测量钙电流和膜电容在一个大型哺乳动物中枢神经突触，萼举行，我们报告的第一次，极低频电磁场严重影响突触传递和可塑性。暴露于ELF-EMF 8至10天显著增加刺激后的钙内流，并促进所有形式的囊泡内吞作用，包括缓慢和快速内吞作用、内吞作用过冲和大量内吞作用，但不影响RRP大小和胞吐作用。暴露于ELF-EMF也增强PTP，这是一种短期可塑性，增加其峰值幅度而不影响其时间进程。我们进一步研究了潜在的机制，发现突触前神经末梢的钙通道表达（包括P/Q、N和R亚型）增强，这是刺激后钙内流增加的原因。因此，我们得出结论，暴露于ELF-EMF有利于囊泡内吞和突触可塑性的钙依赖性的方式，通过增加在神经末梢的钙通道表达。
Accumulating evidence suggests significant biological effects caused by extremely low frequency electromagnetic fields (ELF-EMF). Although exo-endocytosis plays crucial physical and biological roles in neuronal communication, studies on how ELF-EMF regulates this process are scarce. By directly measuring calcium currents and membrane capacitance at a large mammalian central nervous synapse, the calyx of Held, we report for the first time that ELF-EMF critically affects synaptic transmission and plasticity. Exposure to ELF-EMF for 8 to 10 days dramatically increases the calcium influx upon stimulation and facilitates all forms of vesicle endocytosis, including slow and rapid endocytosis, endocytosis overshoot and bulk endocytosis, but does not affect the RRP size and exocytosis. Exposure to ELF-EMF also potentiates PTP, a form of short-term plasticity, increasing its peak amplitude without impacting its time course. We further investigated the underlying mechanisms and found that calcium channel expression, including the P/Q, N, and R subtypes, at the presynaptic nerve terminal was enhanced, accounting for the increased calcium influx upon stimulation. Thus, we conclude that exposure to ELF-EMF facilitates vesicle endocytosis and synaptic plasticity in a calcium-dependent manner by increasing calcium channel expression at the nerve terminal.