Tracheal cartilage regeneration and new bone formation by slow release of bone morphogenetic protein (BMP)-2
Tracheal cartilage regeneration and new bone formation by slow release of bone morphogenetic protein (BMP)-2
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DOI：
10.1097/mat.0b013e31815fd3d3
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发表时间：
2008-01-01
期刊：
影响因子：
4.2
通讯作者：
Yokomise, Hiroyasu
中科院分区：
文献类型：
--
作者：
Igai, Hitoshi;Chang, Sung Soo;Yokomise, Hiroyasu
我们研究了明胶海绵缓慢释放的骨形态发生蛋白（BMP）-2对气管软骨再生的效率。在10个连续气管软骨的中腹侧部分中制作1 cm间隙。在对照组（n = 4）中，所产生的间隙不被处理。明胶组（n = 4），在差距植入普通明胶。在BMP-2组（n = 4）中，植入含有100 μ g BMP-2的明胶。我们分别在植入后1、3、6和12个月对每组中的所有犬实施安乐死，然后对植入部位进行肉眼和显微镜检查。BMP-2组在1个月和12个月时观察到再生的纤维软骨和新形成的骨。在宿主软骨残端观察到再生的软骨，在中间部分观察到新形成的骨。间隙被再生的软骨和新形成的骨填充。在3个月和6个月时，再生的软骨，但不是新形成的骨，是明显的。再生软骨被软骨膜覆盖，与宿主软骨具有连续性。我们通过从明胶中缓慢释放100 μ g BMP-2成功地诱导了犬气管中的软骨再生和新骨形成。
We investigated the efficiency of bone morphogenetic protein (BMP)-2 released slowly from gelatin sponge for tracheal cartilage regeneration. A 1-cm gap was made in the mid-ventral portion of each of 10 consecutive tracheal cartilages. In the control group (n = 4), the resulting gap was left untreated. In the gelatin group (n = 4), plain gelatin was implanted in the gap. In the BMP-2 group (n = 4), gelatin containing 100 mu g BMP-2 was implanted. We euthanatized all dogs in each group at 1, 3, 6, and 12 months after the implantation, respectively, and then examined the implant site macro- and microscopically. in the BMP-2 group, regenerated fibrous cartilage and newly formed bone were observed at 1 and 12 months. Regenerated cartilage was observed at the ends of the host cartilage stumps, with newly formed bone in the middle portion. The gaps were filled with regenerated cartilage and newly formed bone. At 3 and 6 months, regenerated cartilage, but not newly formed bone, was evident. The regenerated cartilage was covered with perichondrium and showed continuity with the host cartilage. We succeeded in inducing cartilage regeneration and new bone formation in canine trachea by slow release of 100 mu g BMP-2 from gelatin.