Construction of Ribonuclease-Decorated Artificial Virus-like Capsid by Peptide Self-assembly
Construction of Ribonuclease-Decorated Artificial Virus-like Capsid by Peptide Self-assembly
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DOI：
10.1021/acs.joc.9b02295
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发表时间：
2020-02-07
影响因子：
3.6
通讯作者：
Inaba, Hiroshi
中科院分区：
文献类型：
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作者：
Matsuura, Kazunori;Ota, Junpei;Inaba, Hiroshi
通过β-环-S-肽的自组装和S-肽与S-蛋白的相互作用，构建了表面修饰核糖核酸酶S（RNase S）的人工病毒样衣壳。β-环-S-肽通过β-环-SBz肽与含Cys的S-肽的天然化学连接合成，所述含Cys的S-肽自组装成大小约47 nm的人工病毒样衣壳。在人工病毒样衣壳上的RNase S的重建提供了在表面上附着小球体的球形组装体，其保留了核糖核酸酶活性。
Artificial virus-like capsids decorated with ribonuclease S (RNase S) on their exterior were constructed by the self-assembly of beta-annulus-S-peptide and the interaction between S-peptide moiety and S-protein. The beta-annulus-S-peptide was synthesized by native chemical ligation of beta-annulus-SBz peptide with Cys-containing S-peptide that self-assembled into artificial virus-like capsids of approximately 47 nm in size. Reconstruction of RNase S on the artificial virus-like capsids afforded spherical assembly attached small spheres on the surface, which retained ribonuclease activity.