GENERATION AND CHARACTERIZATION OF MOUSE MONOCLONAL-ANTIBODIES TO THE MYELIN-ASSOCIATED GLYCOPROTEIN (MAG)
GENERATION AND CHARACTERIZATION OF MOUSE MONOCLONAL-ANTIBODIES TO THE MYELIN-ASSOCIATED GLYCOPROTEIN (MAG)
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DOI：
10.1007/bf00964654
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发表时间：
1985-01-01
影响因子：
4.4
通讯作者：
QUARLES, RH
中科院分区：
文献类型：
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作者：
DOBERSEN, MJ;HAMMER, JA;QUARLES, RH
研制了一组小鼠抗大鼠和人髓鞘相关糖蛋白(MAG)的单抗。正常小鼠对大鼠MAG无反应，大鼠MAG仅在自身免疫的NZB小鼠中成功免疫。所有品系的小鼠对人MAG均有反应。已开发的单抗在Ig类型、对人和/或大鼠MAG的特异性以及对MAG中蛋白质或碳水化合物表位的识别方面有所不同。一般来说，与蛋白质骨架反应的抗体既能识别大鼠MAG，也能识别人MAG，而大量的单抗识别人MAG中不存在的糖决定簇。用单抗对成人脊髓进行免疫细胞化学染色，结果显示轴突周围髓鞘染色，与明确定义的兔多克隆抗MAG血清所产生的髓鞘染色相似。识别人MAG中碳水化合物决定簇的抗体对少突胶质细胞胞浆进行了强烈染色。为进一步研究MAG的化学和生物学特性奠定了基础。
A panel of mouse monoclonal antibodies to rat and human myelin-associated glycoprotein (MAG) was developed. Normal mice were unresponsive to rat MAG, and successful immunization with rat MAG was only achieved in autoimmune NZB mice. All strains of mice were responsive to human MAG. The monoclonal antibodies developed differ with respect to Ig type, their specificity for human and/or rat MAG, and their recognition of protein or carbohydrate epitopes in MAG. In general, the antibodies that react with the protein backbone recognize both rat and human MAG, whereas a large number of the monoclonal antibodies recognize a carbohydrate determinant in human MAG that is not in rat MAG. Immunocytochemical staining of adult human spinal cord with the monoclonal antibodies resulted in periaxonal staining of myelin sheaths similar to that produced by well-defined, rabbit, polyclonal anti-MAG serum. The antibodies recognizing carbohydrate determinants in human MAG strongly stained oligodendrocyte cytoplasm. These monoclonal antibodies will be of value for the further chemical and biological characterization of MAG.