Conservation of histone binding and transcriptional repressor functions in a Schizosaccharomyces pombe Tup1p homolog.
Conservation of histone binding and transcriptional repressor functions in a Schizosaccharomyces pombe Tup1p homolog.
复制标题
裂殖酵母 Tup1p 同源物中组蛋白结合和转录抑制功能的保守。
DOI：
10.1128/mcb.19.12.8461
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发表时间：
1999
影响因子：
5.3
通讯作者：
Harashima,S
中科院分区：
文献类型：
--
作者：
Mukai,Y;Matsuo,E;Roth,SY;Harashima,S
Ssn 6p-Tup 1 p辅阻遏物复合物对酿酒酵母中几个不同基因的调节很重要，并作为辅阻遏物功能的模型。为了研究这些功能在进化上的保守性，我们将与S.从乳酸克鲁维酵母（Kluyveromyceslactis，TUP 1）和粟酒裂殖酵母（Schizosophyomycespombe，tup 11+）中克隆了酿酒酵母TUP 1基因。有趣的是，当K. lactis TUP 1基因与S.酿酒酵母tup 1无效突变，S. pombe tup 11+基因则没有，即使在S.酿酒酵母TUP 1启动子。然而，一个S。pombe Tup 11 p-LexA融合蛋白抑制了相应报告基因的转录，表明该Tup 1 p同源物具有内在抑制活性。此外，含有S.酿酒酵母Tup 1 p和来自S. pombe Tup 11 p是对S.酿酒酵母tup 1突变。本征S. pombe Tup 11 p以补充S. A党对应于不能与S结合。酿酒酵母Ssn 6p。tup 11+的破坏与tup 12+的破坏相结合，tup 12+是S. pombe引起葡萄糖阻遏fbp 1+的缺陷，表明S.粟酒裂殖酵母Tup 1 p同源物在S.粟酒此外，Tup 11 p在体外特异性结合组蛋白H3和H4，表明Tup 1 p相关蛋白的抑制和组蛋白结合功能在物种间是保守的。
The Ssn6p-Tup1p corepressor complex is important to the regulation of several diverse genes in Saccharomyces cerevisiae and serves as a model for corepressor functions. To investigate the evolutionary conservation of these functions, sequences homologous to the S. cerevisiae TUP1 gene were cloned from Kluyveromyces lactis (TUP1) and Schizosaccharomyces pombe (tup11+). Interestingly, while the K. lactis TUP1 gene complemented an S. cerevisiae tup1 null mutation, the S. pombe tup11+ gene did not, even when expressed under the control of the S. cerevisiae TUP1 promoter. However, an S. pombe Tup11p-LexA fusion protein repressed transcription of a corresponding reporter gene, indicating that this Tup1p homolog has intrinsic repressor activity. Moreover, a chimeric protein containing the amino-terminal Ssn6p-binding domain of S. cerevisiae Tup1p and 544 amino acids from the C-terminal region of S. pombe Tup11p complemented the S. cerevisiae tup1 mutation. The failure of native S. pombe Tup11p to complement loss of Tup1p functions in S. cerevisiaecorresponds to an inability to bind to S. cerevisiae Ssn6p in vitro. Disruption of tup11+ in combination with a disruption of tup12+, another TUP1 homolog gene in S. pombe, causes a defect in glucose repression of fbp1+, suggesting that S. pombe Tup1p homologs function as repressors in S. pombe. Furthermore, Tup11p binds specifically to histones H3 and H4 in vitro, indicating that both the repression and histone binding functions of Tup1p-related proteins are conserved across species.