Detection of exon skipping and retained introns in transcription of the human thrombospondin 2 gene
Detection of exon skipping and retained introns in transcription of the human thrombospondin 2 gene
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DOI：
10.1006/bbrc.1999.0812
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发表时间：
1999-06-16
影响因子：
3.1
通讯作者：
Adolph, KW
中科院分区：
文献类型：
--
作者：
Adolph, KW
人血小板反应蛋白2（TSP 2），细胞外糖蛋白的基因的替代转录本，已被确定使用PCR程序。显示外显子跳跃和/或保留内含子的物种被揭示。特别地，表征了其中外显子3已从序列剪接的物种。该转录本从外显子1A至外显子22是完整的。还鉴定了保留内含子IA、内含子1B或内含子2的转录物。在保留内含子的转录本中存在明显的组织特异性差异，此外，还发现了一个既保留内含子1B又跳过外显子3的物种。人类TSP 2基因的这些替代转录本的存在对于理解TSP 2的不同功能和组织特异性表达可能是重要的。(C)北京：科学出版社.
Alternative transcripts of the gene for human thrombospondin 2 (TSP2), an extracellular glycoprotein, have been identified using a PCR procedure. Species that show exon skipping and/or retained introns were revealed. In particular, a species was characterized in which exon 3 has been spliced from the sequence. This transcript is otherwise intact from exon 1A to exon 22. Transcripts which retain intron IA, intron 1B, or intron 2 were also identified. Tissue specific differences were evident in the transcripts with retained introns, In addition, a species which both retained intron 1B and skipped exon 3 was revealed. The existence of these alternative transcripts of the human TSP2 gene could be important for understanding the diverse functions and tissue-specific expression implicated for TSP2. (C) 1999 Academic Press.