Identification of hydrolysable tannins in the reaction zone of Eucalyptus nitens wood by high performance liquid chromatography-electrospray ionisation mass spectrometry
Identification of hydrolysable tannins in the reaction zone of Eucalyptus nitens wood by high performance liquid chromatography-electrospray ionisation mass spectrometry
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DOI：
10.1002/pca.548
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发表时间：
2001-03-01
影响因子：
3.3
通讯作者：
Mohammed, CL
中科院分区：
文献类型：
--
作者：
Barry, KM;Davies, NW;Mohammed, CL
首次详细分析了桉树边材反应区（抗菌防御组织）的酚类成分。给出种植园生长的桉树nitens树木与干腐烂修剪伤口造成的采样和提取物制备健康边材和反应区组织。通过HPLC与ESI-MS的分析表明，健康边材和反应区提取物中存在多种可水解单宁，包括30多种没食子单宁、鞣花单宁和酚类。明确鉴定了八种单宁，包括没食子单宁三-O-没食子酰基-β-D-葡萄糖、四-O-没食子酰基-β-D-葡萄糖和五-O-没食子酰基-β-D-葡萄糖，以及鞣花单宁pedunculagin、tellimagrandin I、紫茎泽兰素、紫茎泽兰素和tellimagrandin II。酚类化合物没食子酸、鞣花酸和儿茶素也被鉴定出来。鞣花单宁（特别是花梗单宁）在反应区中比在健康边材中丰富得多，并且可能有助于反应区作为抗微生物屏障的有效性。版权所有（C）2001约翰威利父子有限公司
The first detailed analysis of the phenolic constituents of the reaction zones (tissue of antimicrobial defence) from the sapwood of a Eucalyptus spp. is presented. Plantation-grown Eucalyptus nitens trees with stem decay resulting from pruning wounds were sampled and extracts were prepared from healthy sapwood and from reaction zone tissue. Analysis by HPLC with ESI-MS revealed that a diverse range of hydrolysable tannins are present in both healthy sapwood and in reaction zone extracts, including over 30 gallotannins, ellagitannins and phenols. Eight tannins were unequivocally identified, including the gallotannins tri-O-galloyl-beta -D-glucose, tetra-O-galloyl-beta -D-glucose and penta-O-galloyl-beta -D-glucose, and the ellagitannins pedunculagin, tellimagrandin I, casuarinin, casuarictin and tellimagrandin II. The phenols gallic acid, ellagic acid and catechin were also identified. The ellagitannins (particularly pedunculagin) are considerably more abundant in the reaction zone than in the healthy sapwood and may contribute to the effectiveness of the reaction zone as an antimicrobial barrier. Copyright (C) 2001 John Wiley & Sons, Ltd.