Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B1 in maize samples
Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B1 in maize samples
复制标题
基于单链可变片段抗体的免疫层析试纸条，用于快速检测玉米样品中的伏马菌素 B-1
DOI：
10.1016/j.foodchem.2020.126546
复制
发表时间：
2020-07-30
期刊：
影响因子：
8.8
通讯作者：
Hammock, Bruce D.
中科院分区：
文献类型：
--
作者：
Ren, Wenjie;Xu, Yang;Hammock, Bruce D.
以伏马菌素B-1（FB 1）为抗原，制备了一种快速、灵敏的单链抗体免疫层析试纸条（ICS）。ICS是基于FB 1和FB 1-BSA之间胶体金标记的scFv的竞争反应，其与羊抗小鼠IgG一起沿着用作捕获试剂，分别固定在测试线和对照线上，在条的硝酸纤维素膜上。ICS的检测限为2.5 ng/mL（25 μ g/kg）FB 1在缓冲液中，和灵敏度是8倍高于单克隆抗体的制备的scFv。用ICS法检测了该单链抗体与常见真菌毒素的交叉反应性，结果表明该单链抗体不与其他真菌毒素发生交叉反应。8个自然污染的玉米样品进行了分析与基于单链抗体的ICS和LC-MS/MS。与条带测定得到的分析结果显示出良好的一致性，通过LC-MS/MS得到的。
A rapid and sensitive immunochromatographic strip (ICS) based on a single-chain variable fragment (scFv) was developed for detecting fumonisin B-1 (FB1). The ICS was based on a competitive reaction for colloidal gold-labeled scFv between FB1 and FB1-BSA, which was used along with sheep anti-mouse IgG as capture reagents immobilized at test and control lines, respectively, on a nitrocellulose membrane of the strip. The limit of detection of the ICS was 2.5 ng/mL (25 mu g/kg) FB1 in buffer, and the sensitivity was eight times higher than that of monoclonal antibodies for the preparation of the scFv. The cross-reactivity of the scFv with common mycotoxins was determined by ICS, the results showed that the scFv were not against other mycotoxins. Eight naturally contaminated maize samples were analyzed with the scFv-based ICS and by LC-MS/MS. The results of analysis obtained with the strip assay showed good agreement with those obtained by LC-MS/MS.