Petrobactin-mediated iron transport in pathogenic bacteria: Coordination chemistry of an unusual 3,4-catecholate/citrate siderophore
Petrobactin-mediated iron transport in pathogenic bacteria: Coordination chemistry of an unusual 3,4-catecholate/citrate siderophore
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DOI：
10.1021/ja077202g
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发表时间：
2008-02-20
影响因子：
15
通讯作者：
Raymond, Kenneth N.
中科院分区：
文献类型：
--
作者：
Abergel, Rebecca J.;Zawadzka, Anna M.;Raymond, Kenneth N.
六齿3，4-儿茶酚酸盐/柠檬酸盐铁载体petrobactin由哺乳动物病原体炭疽芽孢杆菌和蜡状芽孢杆菌产生，显然是为了逃避免疫蛋白铁载体蛋白并介导它们在感染期间的铁获得;它对这些生物体的致病性至关重要。溶液热力学和动力学测量表明，与异羟肟酸单元相比，将3，4-邻苯二酚亚基掺入铁载体结构中并不会增强其对铁的亲和力，但它可以促进从人二铁转铁蛋白中快速去除铁。光反应性柠檬酸铁部分的功能在致病性芽孢杆菌中仍然未被探索;然而，土壤生物枯草芽孢杆菌可以利用铁岩杆菌素及其光产物作为铁源，因此携带这种外源铁载体的特异性摄取系统。
The hexadentats 3,4-catecholate/citrate siderophore petrobactin is produced by the mammalian pathogens Bacillus anthracis and Bacillus cereus apparently to evade the immune protein siderocalin and mediate their iron acquisition during infection; it is essential for the pathogenicity of these organisms. Solution thermodynamic and kinetic measurements show that incorporation of 3,4-catecholate subunits into the siderophore structure does not enhance its affinity for iron as compared to hydroxamate units, but it promotes fast iron removal from human diferric transferrin. The function of the photoreactive ferric citrate moiety remains unexplored in pathogenic Bacilli; however, the soil organism Bacillus subtilis can utilize both ferric petrobactin and its photoproduct as iron sources and therefore carries a specific uptake system for this exogenous siderophore.