Is superoxide demonstration by electron-spin resonance spectroscopy really superoxide?
Is superoxide demonstration by electron-spin resonance spectroscopy really superoxide?
复制标题
电子自旋共振光谱证明的超氧化物真的是超氧化物吗？
作者：
H. Nakazawa;K. Ichimori;Y. Shinozaki;H. Okino;S. Hori
最近的一项研究表明，氧自由基的产生在冷冻夹心肌再灌注可以直接证明使用电子自旋共振波谱（ESR）。然而，结果需要谨慎分析，因为人工部首是这种方法常见的误导性问题。为了测试报告的超氧化物是否是真正的生物存在的自由基或人为的自由基，我们进行了实验，使用隔离，灌注大鼠和兔心脏和开胸犬心脏缺血/再灌注。自由基在77 K下被冷冻捕获，并进行ESR测量。ESR谱显示有四种自由基。其中，两个自由基已被先前声称为超氧化物和氮为中心的自由基被证明是机械产生的人工自由基。这些通过粉碎冷冻样品产生。在无人工制品的样品中，无法检测到超氧化物。心肌天然自由基被鉴定为辅酶Q10-。另一种激进分子的种类尚不清楚
A recent study has indicated that the generation of an oxygen radical in freeze-clamped myocardium on reperfusion can be directly demonstrated using electron-spin resonance spectroscopy (ESR). However, the results need to be analyzed with caution, since artifactual radicals are misleading problems common to this method. To test whether that reported superoxide is truly the biologically existing radical or an artifactual radical, we performed experiments using isolated, perfused rat and rabbit hearts and open-chest canine hearts subjected to ischemia/reperfusion. Radicals were freeze trapped at 77 degrees K, and ESR measurements were made. The ESR spectra exhibited four free radicals. Among these, two radicals which had been previously claimed as superoxide and a nitrogen-centered radical were shown as mechanically yielded artifactual radicals. These were produced by pulverization of the frozen sample. In artifact-free samples, superoxide could not be detected. The radicals native to the myocardium were identified as coenzyme Q10-. and another radical the species of which remains unclear.