Fetal development and neuronal/glial cell specificity of cAMP-dependent protein kinase subunit mRNAs in rat brain.
Fetal development and neuronal/glial cell specificity of cAMP-dependent protein kinase subunit mRNAs in rat brain.
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DOI：
10.1159/000112140
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发表时间：
1991
影响因子：
2.9
通讯作者：
J. Massa;P. S. Walker;D. Moser;R. Fellows;R. Maurer
中科院分区：
文献类型：
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作者：
J. Massa;P. S. Walker;D. Moser;R. Fellows;R. Maurer
所有已知的cAMP在脑内的作用都需要cAMP依赖的蛋白激酶(CAMPdPK)，cAMPdPK由调节性(R)和催化(C)亚基(R2C2)组成。利用脑中已知的所有cAMPdPK亚基亚基(RIα、RIβ、RIIα、RIIβ、Cα、Cβ)的同源cDNA，我们观察到，从妊娠12天到出生，在胎鼠脑中，α亚单位(RIα、RIIα、Cα)的mRNA水平丰富，而β亚单位(RIβ、RIIβ、Cβ)的mRNA水平从检测不到或非常低的水平上升到丰富的水平。此外，虽然在原代神经元和原代神经胶质细胞培养中都有丰富的α亚基mRNA水平，但在原代神经胶质细胞培养中，β亚基mRNA水平很低(Cβ)或检测不到(RIβ，RIIβ)，但在原代神经元培养中却很丰富。因此，在怀孕12天之前，大脑中的cAMP可能只通过神经元和神经胶质前体细胞中的αcAMPdPK亚单位起作用。妊娠12天后，与神经元最终细胞分裂的开始一致，βcAMPdPK亚基也可能主要在神经元中介导cAMP的作用。
All known actions of cAMP in the brain require cAMP-dependent protein kinase (cAMPdPK), which consists of regulatory (R) and catalytic (C) subunits (R2C2). Using homologous rat cDNAs for all known cAMPdPK subunit isoforms found in the brain (RI alpha, RI beta, RII alpha, RII beta, C alpha, C beta) we observe that, in the fetal rat brain from 12 days of gestation to birth, while alpha subunit (RI alpha, RII alpha, C alpha) mRNA levels are abundant, beta subunit (RI beta, RII beta, C beta) mRNA levels increase from undetectable or very low levels to abundant levels. Furthermore, while alpha subunit mRNA levels are abundant in both primary neuronal and primary glial cultures, beta subunit mRNA levels are very low (C beta) or undetectable (RI beta, RII beta) in primary glial cultures, but are abundant in primary neuronal cultures. Thus, prior to about 12 days of gestation, cAMP in the brain may act only via the alpha cAMPdPK subunits in neuronal and glial precursor cells. After 12 days of gestation, coincident with the onset of final cell division in neurons, beta cAMPdPK subunits may also mediate the effects of cAMP predominantly in neurons.