Strikingly Fast Microtubule Sliding in Bundles Formed by Chlamydomonas Axonemal Dynein
Strikingly Fast Microtubule Sliding in Bundles Formed by Chlamydomonas Axonemal Dynein
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DOI：
10.1002/cm.20450
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发表时间：
2010-06-01
期刊：
影响因子：
2.9
通讯作者：
Kamiya, Ritsu
中科院分区：
文献类型：
--
作者：
Aoyama, Susumu;Kamiya, Ritsu
Chlamydomonas axonemal extracts containing outer-arm dynein bundle microtubules when added in the absence of ATP. The bundles dissociate after addition of ATP (Haimo et al., Proc Nail Acad Sci USA 76:5759-5768, 1979). In the present study, we investigated the ATP-induced bundle dissociation process using caged ATP. Application of similar to 0.5 mM ATP induced microtubule sliding at similar to 30 mu m.s(-1), which was 1.5 times faster than the microtubule sliding observed in protease-treated axonemes and five times faster than microtubule gliding on glass surfaces coated with outer-arm dynein. Bundles formed by mutant dynein molecules that lack one of the three heavy chains (HCs) displayed similar high-speed intermicrotubule sliding. These results suggest that Chlamydomonas outer-arm dynein molecules, when aligned, can translocate microtubules at high speed and that the high-speed sliding under load-free conditions does not require the complete set of the three HCs. It is likely that each of the three HCs has the ability to produce highspeed sliding, which should be an important property for their cooperation. (C) 2010 Wiley-Liss, Inc
