SECONDARY STRUCTURE OF 16S-RIBOSOMAL RNA
SECONDARY STRUCTURE OF 16S-RIBOSOMAL RNA
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DOI：
10.1126/science.6163215
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发表时间：
1981-01-01
期刊：
影响因子：
56.9
通讯作者：
WOESE, CR
中科院分区：
文献类型：
--
作者：
NOLLER, HF;WOESE, CR
基于现有化学、酶和比较序列数据的大肠杆菌16S rRNA二级结构模型表明，该模型所规定的约束条件与各种实验观察结果之间具有良好的一致性。碱基配对方案产生的4个主要结构域与在结合核糖体蛋白存在的情况下核酸酶消化后分离的RNA片段密切相关。功能上重要的位点似乎位于未配对的区域，并且在系统发育上高度保守。
A secondary structure model for Escherichia coli 16S rRNA which is based on available chemical, enzymatic and comparative sequence data shows good agreement between constraints dictated by the model and a wide variety of experimental observations. The 4 major structural domains created by the base-pairing scheme correspond closely to RNA fragments isolated after nuclease digestion in the presence of bound ribosomal proteins. Functionally important sites appear to be located in unpaired regions and are phylogenetically highly conserved.