Magnetic labeling of activated microglia in experimental gliomas
Magnetic labeling of activated microglia in experimental gliomas
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DOI：
10.1038/sj.neo.7900176
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发表时间：
2001-11-01
期刊：
影响因子：
4.8
通讯作者：
Zimmer, C
中科院分区：
文献类型：
--
作者：
Fleige, G;Nolte, C;Zimmer, C
小胶质细胞作为中枢神经系统（CNS）的内在免疫效应细胞，在对几乎任何脑病理的应答中发挥非常敏感的关键作用，其中它们被激活至吞噬状态。基于激活的小胶质细胞的特征，我们研究了这些细胞是否可以使用超小超顺磁性氧化铁（USPIOs）与磁共振成像（MRI）可视化。本研究的假设是，MR小胶质细胞可视化不仅可以显示肿瘤的范围，而且可以评估免疫防御状态。在细胞培养实验和大鼠胶质瘤模型中使用USPIO，我们表明小胶质细胞可以被磁性标记。标记的小胶质细胞通过共聚焦显微镜以典型的边界样模式在肿瘤内和周围检测。体外定量研究表明，小胶质细胞内化的USPIO量显著高于肿瘤细胞和星形胶质细胞所掺入的USPIO量。标记的小胶质细胞可以在细胞模型中用MRI检测和定量，并且可以在体内的荷胶质瘤大鼠中看到肿瘤的范围。我们的结论是，磁标记的小胶质细胞提供了一个潜在的工具，磁共振成像的胶质瘤，它反映了肿瘤的形态准确。此外，结果表明，MRI可以产生功能数据的免疫反应的中枢神经系统。
Microglia, as intrinsic immunoeffector cells of the central nervous system (CNS), play a very sensitive, crucial role in the response to almost any brain pathology where they are activated to a phagocytic state. Based on the characteristic features of activated microglia, we investigated whether these cells can be visualized with magnetic resonance imaging (MRI) using ultrasmall superparamagnetic iron oxides (USPIOs). The hypothesis of this study was that MR microglia visualization could not only reveal the extent of the tumor, but also allow for assessing the status of immunologic defense. Using USPIOs in cell culture experiments and in a rat glioma model, we showed that microglia can be labeled magnetically. Labeled microglia are detected by confocal microscopy within and around tumors in a typical border-like pattern. Quantitative in vitro studies revealed that microglia internalize amounts of USPIOs that are significantly higher than those incorporated by tumor cells and astrocytes. Labeled microglia can be detected and quantified with MRI in cell phantoms, and the extent of the tumor can be seen in glioma-bearing rats in vivo. We conclude that magnetic labeling of microglia provides a potential tool for MRI of gliomas, which reflects tumor morphology precisely. Furthermore, the results suggest that MRI may yield functional data on the immunologic reaction of the CNS.