IMMUNOHISTOCHEMICAL LOCALIZATION OF LOW-DENSITY LIPOPROTEIN RECEPTORS IN ADRENAL-GLAND, LIVER, AND INTESTINE
IMMUNOHISTOCHEMICAL LOCALIZATION OF LOW-DENSITY LIPOPROTEIN RECEPTORS IN ADRENAL-GLAND, LIVER, AND INTESTINE
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DOI：
10.1172/jci114245
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发表时间：
1989-09-01
影响因子：
15.9
通讯作者：
COOPER, AD
中科院分区：
文献类型：
--
作者：
FONG, LG;BONNEY, E;COOPER, AD
用免疫组织化学方法研究低密度脂蛋白受体在肾上腺、肝脏和肠道的定位。使用的抗低密度脂蛋白受体抗体被证明是单特异性的，不与横纹肌反应，横纹肌是一种低密度脂蛋白受体水平非常低的组织。同样，大脑皮层只表现出微弱的反应，这是到一个以前被证明有低密度脂蛋白受体的区域。肾上腺与束状带反应强烈，其受体密度高于网状带。在正常肝脏中，LSL受体存在于肝窦膜上，在肝细胞与肝细胞接触的区域稀疏，没有明显的门脉向中心的梯度。低密度脂蛋白受体存在于整个肠道。在空肠，染色最强的部位在绒毛底部，并向上延伸至绒毛顶端。在绒毛底部，受体主要位于基侧膜，但在绒毛顶端有明显的细胞内染色。隐窝染色较淡，十二指肠隐窝染色强度与绒毛区相当。结肠上皮细胞染色较深。这些结果提供了关于低密度脂蛋白受体的细胞和亚细胞定位的新信息，并提出了低密度脂蛋白衍生的胆固醇在新的脂蛋白形成中发挥作用的有趣的可能性。
The localization of LDL receptors in adrenal gland, liver, and intestine was studied using immunohistochemistry. The anti-LDL receptor antibody used was shown to be monospecific and did not react with striated muscle, a tissue which has a very low level of LDL receptors. Similarly, cerebral cortex showed only faint reactivity and that was to an area previously demonstrated to have LDL receptors. Adrenal gland was intensely reactive with the zona fasciculata, having a greater density of receptors than the zona reticularis. In normal liver, LSL receptors were present on the sinusoidal membranes and were sparse in the areas of hepatocyte-to-hepatocyte contact without an obvious portal to central gradient. LDL receptors were present throughout the intestine. In jejunum, staining was most intense at the base of the villus and extended up toward the villus tip. At the base of the villus, the receptor was primarily at the basal lateral membrane, but toward the villus tip, there was appreciable intracellular staining. Staining in crypts was more faint; in duodenum, staining in crypts equaled that in the villus region in intensity. In colon, there was intense staining throughtout the epithelial cells. These results provide new information about the cellular and subcellular localization of LDL receptors and raise the interesting possibility that there is a role for LDL-derived cholesterol in new lipoprotein formation.