Plant Raf-like kinases regulate the mRNA population upstream of ABA-unresponsive SnRK2 kinases under drought stress
Plant Raf-like kinases regulate the mRNA population upstream of ABA-unresponsive SnRK2 kinases under drought stress
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DOI：
10.1038/s41467-020-15239-3
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发表时间：
2020-03-13
影响因子：
16.6
通讯作者：
Yamaguchi-Shinozaki, Kazuko
中科院分区：
文献类型：
--
作者：
Soma, Fumiyuki;Takahashi, Fuminori;Yamaguchi-Shinozaki, Kazuko
SNF 1相关蛋白激酶2（SnRK 2）是调节植物对干旱和高盐等渗透胁迫适应性反应的关键调节因子。亚类III SnRK 2作为脱落酸（阿坝）信号传导的中枢调节剂发挥作用，并协调ABA调节的对渗透胁迫的适应性反应。种子植物已经获得了其他类型的渗透胁迫激活，但ABA无反应的亚类I SnRK 2，调节mRNA的衰减，促进植物生长在渗透胁迫下。与III类SnRK 2相反，I类SnRK 2响应于渗透胁迫而快速激活的调控机制仍然难以捉摸。在这里，我们报告三个B4 Raf-like MAP激酶激酶激酶激酶（MAPKKKs）磷酸化和激活亚类I SnRK 2在渗透胁迫下。转录组分析表明，这些MAPKKK下游基因与I类SnRK 2在渗透胁迫下调控的基因有很大的重叠，表明这些MAPKKK是I类SnRK 2的上游因子，直接被渗透胁迫激活。
SNF1-related protein kinases 2 (SnRK2s) are key regulators governing the plant adaptive responses to osmotic stresses, such as drought and high salinity. Subclass III SnRK2s function as central regulators of abscisic acid (ABA) signalling and orchestrate ABA-regulated adaptive responses to osmotic stresses. Seed plants have acquired other types of osmotic stress-activated but ABA-unresponsive subclass I SnRK2s that regulate mRNA decay and promote plant growth under osmotic stresses. In contrast to subclass III SnRK2s, the regulatory mechanisms underlying the rapid activation of subclass I SnRK2s in response to osmotic stress remain elusive. Here, we report that three B4 Raf-like MAP kinase kinase kinases (MAPKKKs) phosphorylate and activate subclass I SnRK2s under osmotic stress. Transcriptome analyses reveal that genes downstream of these MAPKKKs largely overlap with subclass I SnRK2-regulated genes under osmotic stress, which indicates that these MAPKKKs are upstream factors of subclass I SnRK2 and are directly activated by osmotic stress.